Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2838434..2839071 Replicon chromosome
Accession NZ_CP096840
Organism Mycobacterium tuberculosis variant bovis strain Mb2269

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag M1780_RS13320 Protein ID WP_003413180.1
Coordinates 2838434..2838829 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag M1780_RS13325 Protein ID WP_003413183.1
Coordinates 2838826..2839071 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1780_RS13280 (M1780_13275) 2834519..2835178 + 660 WP_003900846.1 LppA family lipoprotein -
M1780_RS13285 (M1780_13280) 2835175..2835834 + 660 WP_003900847.1 LppA family lipoprotein -
M1780_RS13290 (M1780_13285) 2835831..2836493 + 663 WP_003900848.1 LppA family lipoprotein -
M1780_RS13295 (M1780_13290) 2836490..2836768 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
M1780_RS13300 (M1780_13295) 2836861..2837274 + 414 WP_003413164.1 PIN domain nuclease -
M1780_RS13305 (M1780_13300) 2837313..2837570 + 258 WP_003413167.1 CopG family transcriptional regulator -
M1780_RS13310 (M1780_13305) 2837567..2837944 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
M1780_RS13315 (M1780_13310) 2837960..2838334 - 375 WP_003413177.1 hypothetical protein -
M1780_RS13320 (M1780_13315) 2838434..2838829 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
M1780_RS13325 (M1780_13320) 2838826..2839071 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
M1780_RS13330 (M1780_13325) 2839482..2839901 - 420 WP_003413190.1 A24 family peptidase -
M1780_RS13335 (M1780_13330) 2839913..2840721 - 809 Protein_2632 shikimate dehydrogenase -
M1780_RS13340 (M1780_13335) 2840718..2841971 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
M1780_RS13345 (M1780_13340) 2841964..2842476 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T243597 WP_003413180.1 NZ_CP096840:c2838829-2838434 [Mycobacterium tuberculosis variant bovis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T243597 NZ_OX419557:c1329348-1328602 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTATATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTAGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAGCCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT243597 WP_003413183.1 NZ_CP096840:c2839071-2838826 [Mycobacterium tuberculosis variant bovis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT243597 NZ_OX419557:c1328602-1328432 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References