Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2837313..2837944 Replicon chromosome
Accession NZ_CP096840
Organism Mycobacterium tuberculosis variant bovis strain Mb2269

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag M1780_RS13310 Protein ID WP_003413174.1
Coordinates 2837567..2837944 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag M1780_RS13305 Protein ID WP_003413167.1
Coordinates 2837313..2837570 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1780_RS13270 (M1780_13265) 2832571..2832885 + 315 WP_009937839.1 hypothetical protein -
M1780_RS13275 (M1780_13270) 2833181..2834392 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
M1780_RS13280 (M1780_13275) 2834519..2835178 + 660 WP_003900846.1 LppA family lipoprotein -
M1780_RS13285 (M1780_13280) 2835175..2835834 + 660 WP_003900847.1 LppA family lipoprotein -
M1780_RS13290 (M1780_13285) 2835831..2836493 + 663 WP_003900848.1 LppA family lipoprotein -
M1780_RS13295 (M1780_13290) 2836490..2836768 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
M1780_RS13300 (M1780_13295) 2836861..2837274 + 414 WP_003413164.1 PIN domain nuclease -
M1780_RS13305 (M1780_13300) 2837313..2837570 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
M1780_RS13310 (M1780_13305) 2837567..2837944 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
M1780_RS13315 (M1780_13310) 2837960..2838334 - 375 WP_003413177.1 hypothetical protein -
M1780_RS13320 (M1780_13315) 2838434..2838829 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
M1780_RS13325 (M1780_13320) 2838826..2839071 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
M1780_RS13330 (M1780_13325) 2839482..2839901 - 420 WP_003413190.1 A24 family peptidase -
M1780_RS13335 (M1780_13330) 2839913..2840721 - 809 Protein_2632 shikimate dehydrogenase -
M1780_RS13340 (M1780_13335) 2840718..2841971 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
M1780_RS13345 (M1780_13340) 2841964..2842476 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T243596 WP_003413174.1 NZ_CP096840:2837567-2837944 [Mycobacterium tuberculosis variant bovis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T243596 NZ_OX419557:517936-518286 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT243596 WP_003413167.1 NZ_CP096840:2837313-2837570 [Mycobacterium tuberculosis variant bovis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT243596 NZ_OX419557:517650-517931 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References