Detailed information of TA system TA243594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2822318..2822958
• Replicon: chromosome
• Accession: NZ_CP096840
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2269

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: M1780_RS13210
• Protein ID: WP_003412970.1
• Coordinates: 2822318..2822737 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: M1780_RS13215
• Protein ID: WP_003412975.1
• Coordinates: 2822734..2822958 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1780_RS13180 (M1780_13175)	2817903..2818625	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
M1780_RS13185 (M1780_13180)	2819142..2819369	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
M1780_RS13190 (M1780_13185)	2819366..2819767	+	402	WP_003412963.1	PIN domain-containing protein	-
M1780_RS13195 (M1780_13190)	2819802..2820722	-	921	WP_003412965.1	restriction endonuclease	-
M1780_RS13200 (M1780_13195)	2821063..2821269	-	207	WP_234712436.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
M1780_RS13205 (M1780_13200)	2821367..2822317	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
M1780_RS13210 (M1780_13205)	2822318..2822737	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1780_RS13215 (M1780_13210)	2822734..2822958	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
M1780_RS13220 (M1780_13215)	2822989..2825832	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
M1780_RS13225 (M1780_13220)	2825904..2826305	-	402	WP_003412981.1	hypothetical protein	-
M1780_RS13230 (M1780_13225)	2826305..2826775	-	471	WP_003412985.1	transcription antitermination factor NusB	-
M1780_RS13235 (M1780_13230)	2826778..2827341	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T243594 WP_003412970.1 NZ_CP096840:c2822737-2822318 [Mycobacterium tuberculosis variant bovis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T243594 NZ_OX419555:517937-518287 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT243594 WP_003412975.1 NZ_CP096840:c2822958-2822734 [Mycobacterium tuberculosis variant bovis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT243594 NZ_OX419555:517651-517932 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09