Detailed information of TA system TA243589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2240505..2241131
• Replicon: chromosome
• Accession: NZ_CP096840
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2269

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: M1780_RS10505
• Protein ID: WP_003410075.1
• Coordinates: 2240733..2241131 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: M1780_RS10500
• Protein ID: WP_019283586.1
• Coordinates: 2240505..2240732 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1780_RS10490 (M1780_10490)	2238544..2238888	-	345	WP_003410065.1	ferredoxin family protein	-
M1780_RS10495 (M1780_10495)	2239077..2240246	-	1170	WP_003899126.1	ATP-binding protein	-
M1780_RS10500 (M1780_10500)	2240505..2240732	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1780_RS10505 (M1780_10505)	2240733..2241131	+	399	WP_003410075.1	PIN domain nuclease	Toxin
M1780_RS10510 (M1780_10510)	2241314..2241745	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
M1780_RS10515 (M1780_10515)	2241846..2242280	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
M1780_RS10520 (M1780_10520)	2242717..2242896	-	180	Protein_2079	hypothetical protein	-
M1780_RS10525 (M1780_10525)	2242984..2243604	+	621	WP_003410086.1	IS110 family transposase	-
M1780_RS10530 (M1780_10530)	2243558..2244148	+	591	WP_003899131.1	IS110 family transposase	-
M1780_RS10535 (M1780_10535)	2244276..2245532	-	1257	WP_003410095.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T243589 WP_003410075.1 NZ_CP096840:2240733-2241131 [Mycobacterium tuberculosis variant bovis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T243589 NZ_OX419554:566216-566566 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT243589 WP_019283586.1 NZ_CP096840:2240505-2240732 [Mycobacterium tuberculosis variant bovis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT243589 NZ_OX419554:565930-566211 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4CHG