Detailed information of TA system TA243575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1382918..1383606
• Replicon: chromosome
• Accession: NZ_CP096840
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2269

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: M1780_RS06620
• Protein ID: WP_003406304.1
• Coordinates: 1383175..1383606 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: M1780_RS06615
• Protein ID: WP_003406302.1
• Coordinates: 1382918..1383178 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1780_RS06595 (M1780_06590)	1378495..1379319	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
M1780_RS06600 (M1780_06595)	1379324..1380505	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
M1780_RS06605 (M1780_06600)	1380582..1381682	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
M1780_RS06610 (M1780_06605)	1381853..1382842	+	990	WP_003406301.1	malate dehydrogenase	-
M1780_RS06615 (M1780_06610)	1382918..1383178	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
M1780_RS06620 (M1780_06615)	1383175..1383606	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1780_RS06625 (M1780_06620)	1383629..1385315	-	1687	Protein_1307	PE family protein	-
M1780_RS06630 (M1780_06625)	1385495..1386355	+	861	WP_003406306.1	glycine betaine ABC transporter substrate-binding protein	-
M1780_RS06635 (M1780_06630)	1386436..1387266	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
M1780_RS06640 (M1780_06635)	1387323..1387616	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
M1780_RS06645 (M1780_06640)	1387613..1387882	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T243575 WP_003406304.1 NZ_CP096840:1383175-1383606 [Mycobacterium tuberculosis variant bovis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T243575 NZ_OX419519:3639554-3639820 [Kitasatospora sp. DSM 114396]
GTGAAGATCCGGTTCACTGACGGCGGCTGGGCCGACTACCTCTACTGGCAGGCGAACGACCGCCGCCTGCTCAAGCGCAT
CAACCAGTTGATCGAGGACATCCGCCGCAACGGCCACGAAGGCATCGGCAAACCCGAACCACTGCGCCACGAACTCGCCG
GGGCGTGGTCCCGTCGGATCGACCAGGAACACCGCCTGGTCTACGTCCTCGACGAGCCGGCGGACACCGTGTGCATCATT
GCCTGCCGGTACCACTACAGCAAGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT243575 WP_003406302.1 NZ_CP096840:1382918-1383178 [Mycobacterium tuberculosis variant bovis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT243575 NZ_OX419519:3639279-3639557 [Kitasatospora sp. DSM 114396]
ATGAAGACGATGAGCGCGACTGAGCTCCGCAGCAACCTCGCCGCCGCCCTCGACTCCGTCGAGGACGACGCCGAGGAGCT
CATCATCACCCGAGCCGGCCACGAGCCGCTGGTCGTGGTCTCCCTGGCTGAGTACGCCTCGCTCCGCGAGACCGACTACC
TGCTGCGGTCTCCTGCCAACGCCGAGCACCTGCGCCAGTCCCTCCGGGATTACCAGGAAGGCCGGATGCAGAGCCGCGAA
CTGATCGACCCCGAGGACGTCACCGAGCAGTCCGCGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6