Detailed information of TA system TA243574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1238116..1238684
• Replicon: chromosome
• Accession: NZ_CP096840
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2269

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: M1780_RS05945
• Protein ID: WP_003405865.1
• Coordinates: 1238310..1238684 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: M1780_RS05940
• Protein ID: WP_003405863.1
• Coordinates: 1238116..1238313 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1780_RS05920 (M1780_05920)	1234157..1234795	-	639	WP_003898730.1	lipid droplet-associated protein	-
M1780_RS05925 (M1780_05925)	1234885..1235892	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
M1780_RS05930 (M1780_05930)	1235909..1236892	-	984	WP_003898731.1	hypothetical protein	-
M1780_RS05935 (M1780_05935)	1236955..1238028	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
M1780_RS05940 (M1780_05940)	1238116..1238313	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1780_RS05945 (M1780_05945)	1238310..1238684	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1780_RS05950 (M1780_05950)	1238887..1239585	+	699	WP_010950494.1	hypothetical protein	-
M1780_RS05955 (M1780_05955)	1239703..1239888	+	186	WP_003901093.1	hypothetical protein	-
M1780_RS05960 (M1780_05960)	1239815..1240090	-	276	WP_003405867.1	hypothetical protein	-
M1780_RS05965 (M1780_05965)	1240333..1240656	+	324	WP_003405871.1	putative quinol monooxygenase	-
M1780_RS05970 (M1780_05970)	1240671..1241369	-	699	WP_031646953.1	hypothetical protein	-
M1780_RS05975 (M1780_05975)	1241403..1241612	+	210	WP_003911400.1	hypothetical protein	-
M1780_RS05980 (M1780_05980)	1241564..1242334	-	771	Protein_1178	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T243574 WP_003405865.1 NZ_CP096840:1238310-1238684 [Mycobacterium tuberculosis variant bovis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T243574 NZ_OX411214:c60873-59920 [Vibrio campbellii]
ATGGAAAAACTAAAACAACAATTCCTTGAGGTTTCCGATGCGCTTGAGCTCGGAAACCCAGCCATTATTGAAAAAGATTA
CTGGGTCGTCGCCTTACTTAGCGAATTAGCGAAGGTATCGCCTGTGAATCATCAAATGGTTTTTTCTGGGGGGACTGCGC
TAGCCAAGTCCAACGTTAAAATCCTGAGAATGTCAGAAGATGTAGATATAAAGCTCATTCCCCATCAAGAATTTGGTGAG
CTGAGTCGCGGCCAAAAGAAAGCCGCCCGTAAGACATGCATTAACGAGATTGAAATGGCGATCACTCAAACAGAGCGATT
TACCGTTGAGTCGCGTCTCGTTCGTGATGAATATCGCTATGTTGAGTTTGAACTCAGATACCCTCAGCAGTTTAGCCAAG
CGCCATGTCTTCGCCCCATCATTAAACTTGAGTTAATAGAGACGATCCCTCTACTGGATTTAGAACCACGGCCGATCCAA
TCATTGGTAGCGGAACTGTATCGACAGCCACAAGAAGTAAAAGCGTTTGATTGCGTCTCTATTCAAGCGACCTTGGTGGA
GAAAGTGATTTCAATGTTGCGTCGTACCATGTCAGTAAAGCGCGATACGCAACGTAAGGACGATGCCACGCTTGTACGCC
ACATTTATGATGTTTACTGCATTTTAAAGACCGAAGCACAATTAGAAAACTTCGACCTTACAGCAATGACAGCGTTATTT
AACACGGTATTGAAGGAAGATATCGATCGCTTTGGTAATCAACACACTGAGTTTGTTGCTAACCCTAAACAAGAGCTACA
GCTCGGGCTAAAAGAACTTGAAGATAACCCAGTTTTTCGCCAGCGCTTTCAGGACTTTGTTACCCCTATGGTGTTTAACA
CCGAGCCTCATGATTTTGATACCTGCTTTGCCTCGTTTAAACGCGTTGCGCAGTCGTTAATTAAAACAATCTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT243574 WP_003405863.1 NZ_CP096840:1238116-1238313 [Mycobacterium tuberculosis variant bovis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT243574 NZ_OX411214:c61279-60875 [Vibrio campbellii]
ATGACAGCTGTTGAACGCGTTTATCAAAAGATAAAGCGCTCAAGACGTTATGTTTTTGAGCGCAAAGACTTTGATGGTTT
TGCTAGCTACGACCAAATCGGTCGAGCGTTAAGGCAGTTAGTTAAGCAAGGTGAGTTAATTAAACTTGGCTACGGTCTAT
ACACAAAAGCAAGAATGAATAGTCTAACCGGCAGACCCATGCCCACAAACCCTGGCGGAAGTGATGCCCTGATGCGTGAA
ATTTTAAAGATGAAAGGGGTCGATTTTGAAATGGACAAACTGTCTTTACAAAGCCTATCTGGTAAAAGCACCCAAATCCC
ATCCTCCATTCAATACTCGTGGAACCATAAACAGTTTAATCGCAAGTTAGCTGTCGGTAACCGAGTCTTAAACTCACCGA
GTTAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07