Detailed information of TA system TA243565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 751731..752364
• Replicon: chromosome
• Accession: NZ_CP096840
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2269

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQD6
• Locus tag: M1780_RS03470
• Protein ID: WP_003403365.1
• Coordinates: 751731..752114 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: M1780_RS03475
• Protein ID: WP_003403368.1
• Coordinates: 752209..752364 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1780_RS03445 (M1780_03445)	747023..747559	+	537	WP_003403341.1	50S ribosomal protein L10	-
M1780_RS03450 (M1780_03450)	747596..747988	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
M1780_RS03455 (M1780_03455)	747981..748676	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
M1780_RS03460 (M1780_03460)	748747..750252	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
M1780_RS03465 (M1780_03465)	750333..751343	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
M1780_RS03470 (M1780_03470)	751731..752114	-	384	WP_003403365.1	ribonuclease VapC6	Toxin
M1780_RS03475 (M1780_03475)	752209..752364	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1780_RS03480 (M1780_03480)	752440..753156	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
M1780_RS03485 (M1780_03485)	753432..753740	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
M1780_RS03490 (M1780_03490)	753727..753972	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
M1780_RS03495 (M1780_03495)	754082..754519	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
M1780_RS03500 (M1780_03500)	754516..754770	-	255	WP_003911263.1	antitoxin VapB7	-
M1780_RS03505 (M1780_03505)	754884..757247	+	2364	WP_003403397.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13959.73 Da
• Isoelectric Point: 6.0803

>T243565 WP_003403365.1 NZ_CP096840:c752114-751731 [Mycobacterium tuberculosis variant bovis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T243565 NZ_OX365700:c122211-121915 [Nitrospira sp. DNF]
GTGTTTACCGCCGCGGTGCGGCGTCAGCTAGACGACGAGAGCTATCGGGCGCTGCAATTGGCATTGTTGCTGCGGCCCAC
GCAAGGCCCCATCATTCAAGGTGGCGCGGGACTGCGCAAACTCCGATGGGCGGCGGAGGGGCGAGGCAAACGAGGCGGGG
TCCGCCTCATCTACTATTGGGAGCCGGCAAGCCAGACGTTTTATATGTTGTACCTCTATGCAAAGAACGAGCAGGGCGAC
CTCACGGCAGACCAACTCAAGGTGTTGGCCAAGCTCGTTCGGAGGGAATTCTCATGA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT243565 WP_003403368.1 NZ_CP096840:c752364-752209 [Mycobacterium tuberculosis variant bovis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT243565 NZ_OX365700:c121918-121607 [Nitrospira sp. DNF]
ATGAAGGACACGGCATTTCAAGAGCTGCTGACCAGCATCCGGCAAGCCGGACGCATTCGTCGAGGCACGCTGAAGCCTGC
TCGCGTCACCACCTTTCGTCCGGCCGATGTGAAGAGCATCCGGGAGAAGCTCAAGGCATCGCAGGCCGAATTTGCCTTAA
TGATCGGTGTCAGCATCGCGACGCTCCGTAACTGGGAACAGGGACGGCGAAGGCCTGAAGGTCCGGCGCTCGCTCTACTT
CGCGTCGCAGCTCGCAATCCCCGTGCAGTGGCCGAGGCGTTACATCGCCCACCGAGAAAAGGCGCAGCCTAA

Structures

Toxin

• AlphaFold DB: G0TQD6

Antitoxin

• AlphaFold DB: A0A7U4BSF8