Detailed information of TA system TA243560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 695377..696023
• Replicon: chromosome
• Accession: NZ_CP096840
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2269

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: M1780_RS03145
• Protein ID: WP_003403137.1
• Coordinates: 695377..695790 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: M1780_RS03150
• Protein ID: WP_003403139.1
• Coordinates: 695787..696023 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1780_RS03125 (M1780_03125)	691460..693010	+	1551	WP_003403119.1	MlaD family protein	-
M1780_RS03130 (M1780_03130)	693062..693454	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
M1780_RS03135 (M1780_03135)	693451..693708	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
M1780_RS03140 (M1780_03140)	693891..695126	-	1236	WP_003403128.1	ATP-binding protein	-
M1780_RS03145 (M1780_03145)	695377..695790	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1780_RS03150 (M1780_03150)	695787..696023	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
M1780_RS03155 (M1780_03155)	696127..696633	-	507	WP_003900973.1	two component system sensor kinase HK1	-
M1780_RS03160 (M1780_03160)	696747..697277	-	531	WP_003917320.1	two component system sensor kinase HK2	-
M1780_RS03165 (M1780_03165)	697261..697956	-	696	WP_197044064.1	two-component system response regulator TcrA	-
M1780_RS03170 (M1780_03170)	698079..698390	+	312	WP_003403164.1	hypothetical protein	-
M1780_RS03175 (M1780_03175)	698462..699412	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
M1780_RS03180 (M1780_03180)	699653..700237	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
M1780_RS03185 (M1780_03185)	700239..700949	+	711	Protein_631	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T243560 WP_003403137.1 NZ_CP096840:c695790-695377 [Mycobacterium tuberculosis variant bovis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T243560 NZ_OX359241:c2605555-2605259 [Alteromonas macleodii]
ATGGCTGAAATAGTATGGACAGAACCTGCTCTAAATGACTTGGACAAGATTGCTGAATACATAGCGGTATCTAATTTAGT
TGCGGCAAAGAAACTGGTTAAAGAAGTCTTCGCCAAAGTAGACATGCTTGAGGAATATCTTAAATCAGGAAAGTCAGTCT
CTGAATTACCATCTCTAAATTACCGCGAGCTTTATGTGAAGCCCTGTCGAATTTTTTATAAATTTGAGGACAACAAAGCC
TTTATTTTGCATGTAATGAGGCAAGAACAAGATCTTAGGCGTTTTTTATTAGATTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT243560 WP_003403139.1 NZ_CP096840:c696023-695787 [Mycobacterium tuberculosis variant bovis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT243560 NZ_OX359241:c2605800-2605543 [Alteromonas macleodii]
GTGAAAGTAGAATTAGTTACTACATTAAAGAGACAAGCCACAAAAATTTTGCAAGACCTGCACACTTCAAAAGAGCCTGT
TTTGATTACTGAGCATGGTCAGCCATCGGCTTACTTAGTTGATGTTGATGATTATGAGATGATGCAAAAGCGCATGTCGA
TTCTGGAAGGTATCGCAAAAGGTGAGCAGGCAATAAGAAATGATGACGTTTTAACTAACTTGCAGGCCAAAGAGAAAATG
GATAAATGGCTGAAATAG

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89