Detailed information of TA system TA243555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 361642..362213
• Replicon: chromosome
• Accession: NZ_CP096840
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2269

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: M1780_RS01580
• Protein ID: WP_003401560.1
• Coordinates: 361642..361944 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: M1780_RS01585
• Protein ID: WP_003401563.1
• Coordinates: 361992..362213 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1780_RS01560 (M1780_01560)	357066..357869	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
M1780_RS01565 (M1780_01565)	357879..359276	-	1398	WP_003401544.1	sulfatase	-
M1780_RS01570 (M1780_01570)	359455..361275	+	1821	WP_003401546.1	PE family protein	-
M1780_RS01575 (M1780_01575)	361418..361645	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
M1780_RS01580 (M1780_01580)	361642..361944	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
M1780_RS01585 (M1780_01585)	361992..362213	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
M1780_RS01590 (M1780_01590)	362210..362635	+	426	WP_003401566.1	PIN domain nuclease	-
M1780_RS01595 (M1780_01595)	362771..363403	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
M1780_RS01600 (M1780_01600)	363400..364308	+	909	WP_003900117.1	protochlorophyllide reductase	-
M1780_RS01605 (M1780_01605)	364316..364906	-	591	WP_234712434.1	hypothetical protein	-
M1780_RS01610 (M1780_01610)	364899..364949	-	51	Protein_318	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T243555 WP_003401560.1 NZ_CP096840:361642-361944 [Mycobacterium tuberculosis variant bovis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T243555 NZ_OX359237:c609606-609343 [Alteromonas macleodii]
ATGAGTAACAGGTTGTTATCATGGACTGATGAAGCTTGGAATAGTTACGTTTATTGGCAGACTCAAGACAAGAAAACCTT
GAAACGTATCAATAAGTTAATTTCGGATGTAAAGTGTTCTCCATTCGAAGGAATTGGAAAGCCTGAACCTCTTAAAGAAA
ATCTTTCAGGTTTCTGGTCTCGCAGAATAGATGACACAAACCGTTTGGTATATGCCGTGGACGATACCGCAATTACGGTT
ATCTCGTGTCGTTACCATTACTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT243555 WP_003401563.1 NZ_CP096840:361992-362213 [Mycobacterium tuberculosis variant bovis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT243555 NZ_OX359237:c609853-609599 [Alteromonas macleodii]
ATGAGAATAGTCTCTTTTACCGAAGCACGAAATGGTCTAAAAGCCGTTTTGGACAATGTTATTAACGATGCAGATACAAC
AGTAATCACTCGTCGTGACTCTAAAGATGCTGTTGTAATGTCATTAGATTACTACAACAGCCTTATGGAAACTGTTCATC
TACTTCGTTCTCCAGCGAACGCTCAACACTTAAATCGTTCAATAGCACAGTTTAAAGCGGCAAAAACGACCCAGCGAGAA
CTTATTGATGAGTAA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48