Detailed information of TA system TA243536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2827386..2828026
• Replicon: chromosome
• Accession: NZ_CP096839
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2487

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: M1779_RS13250
• Protein ID: WP_003412970.1
• Coordinates: 2827386..2827805 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: M1779_RS13255
• Protein ID: WP_003412975.1
• Coordinates: 2827802..2828026 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1779_RS13220 (M1779_13220)	2822971..2823693	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
M1779_RS13225 (M1779_13225)	2824210..2824437	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
M1779_RS13230 (M1779_13230)	2824434..2824835	+	402	WP_003412963.1	PIN domain-containing protein	-
M1779_RS13235 (M1779_13235)	2824870..2825790	-	921	WP_003412965.1	restriction endonuclease	-
M1779_RS13240 (M1779_13240)	2826131..2826337	-	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
M1779_RS13245 (M1779_13245)	2826435..2827385	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
M1779_RS13250 (M1779_13250)	2827386..2827805	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1779_RS13255 (M1779_13255)	2827802..2828026	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
M1779_RS13260 (M1779_13260)	2828057..2830900	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
M1779_RS13265 (M1779_13265)	2830972..2831373	-	402	WP_003412981.1	hypothetical protein	-
M1779_RS13270 (M1779_13270)	2831373..2831843	-	471	WP_003412985.1	transcription antitermination factor NusB	-
M1779_RS13275 (M1779_13275)	2831846..2832409	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T243536 WP_003412970.1 NZ_CP096839:c2827805-2827386 [Mycobacterium tuberculosis variant bovis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T243536 NZ_OX359176:53925-54407 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT243536 WP_003412975.1 NZ_CP096839:c2828026-2827802 [Mycobacterium tuberculosis variant bovis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT243536 NZ_OX359176:53671-53937 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09