Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2308666..2309302 Replicon chromosome
Accession NZ_CP096839
Organism Mycobacterium tuberculosis variant bovis strain Mb2487

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag M1779_RS10795 Protein ID WP_003410654.1
Coordinates 2308892..2309302 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag M1779_RS10790 Protein ID WP_003410651.1
Coordinates 2308666..2308899 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1779_RS10775 (M1779_10780) 2304123..2304515 + 393 WP_229303073.1 metal ABC transporter permease -
M1779_RS10780 (M1779_10785) 2304516..2304920 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
M1779_RS10785 (M1779_10790) 2305004..2308588 - 3585 WP_010950652.1 cobaltochelatase subunit CobN -
M1779_RS10790 (M1779_10795) 2308666..2308899 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
M1779_RS10795 (M1779_10800) 2308892..2309302 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
M1779_RS10800 (M1779_10805) 2309286..2310377 + 1092 WP_003900454.1 precorrin-3B synthase -
M1779_RS10805 (M1779_10810) 2310387..2311013 + 627 WP_003410658.1 precorrin-8X methylmutase -
M1779_RS10810 (M1779_10815) 2311010..2312536 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
M1779_RS10815 (M1779_10820) 2312482..2313705 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T243532 WP_003410654.1 NZ_CP096839:2308892-2309302 [Mycobacterium tuberculosis variant bovis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T243532 NZ_OX359175:c4849789-4849505 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT243532 WP_003410651.1 NZ_CP096839:2308666-2308899 [Mycobacterium tuberculosis variant bovis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT243532 NZ_OX359175:c4850021-4849779 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGGTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References