Detailed information of TA system TA243521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1752047..1752660
• Replicon: chromosome
• Accession: NZ_CP096839
• Organism: Mycobacterium tuberculosis variant bovis strain Mb2487

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: M1779_RS08225
• Protein ID: WP_003407786.1
• Coordinates: 1752256..1752660 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: M1779_RS08220
• Protein ID: WP_009935474.1
• Coordinates: 1752047..1752250 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1779_RS08205 (M1779_08215)	1747311..1750214	+	2904	WP_273585722.1	MMPL family transporter	-
M1779_RS08210 (M1779_08220)	1750224..1750670	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
M1779_RS08215 (M1779_08225)	1750705..1751994	+	1290	WP_003407781.1	threonine ammonia-lyase	-
M1779_RS08220 (M1779_08230)	1752047..1752250	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M1779_RS08225 (M1779_08235)	1752256..1752660	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
M1779_RS08230 (M1779_08240)	1752677..1754418	-	1742	Protein_1623	malto-oligosyltrehalose trehalohydrolase	-
M1779_RS08235 (M1779_08245)	1754411..1756706	-	2296	Protein_1624	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T243521 WP_003407786.1 NZ_CP096839:1752256-1752660 [Mycobacterium tuberculosis variant bovis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T243521 NZ_OX359175:324280-324648 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT243521 WP_009935474.1 NZ_CP096839:1752047-1752250 [Mycobacterium tuberculosis variant bovis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT243521 NZ_OX359175:324062-324283 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46