Detailed information of TA system TA024350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1838656..1839294
• Replicon: chromosome
• Accession: NC_013364
• Organism: Escherichia coli O111:H- str. 11128

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ECO111_RS09610
• Protein ID: WP_000813794.1
• Coordinates: 1838656..1838832 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECO111_RS09615
• Protein ID: WP_001270286.1
• Coordinates: 1838878..1839294 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO111_RS09590	1834281..1835489	-	1209	WP_071532666.1	BenE family transporter YdcO	-
ECO111_RS09595	1835542..1836078	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
ECO111_RS09600	1836151..1838112	+	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ECO111_RS09605	1838204..1838434	-	231	WP_000494244.1	YncJ family protein	-
ECO111_RS09610	1838656..1838832	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ECO111_RS09615	1838878..1839294	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ECO111_RS09620	1839373..1840779	+	1407	WP_000760637.1	PLP-dependent aminotransferase family protein	-
ECO111_RS09625	1841024..1842169	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
ECO111_RS09630	1842187..1843200	+	1014	WP_000220394.1	ABC transporter ATP-binding protein	-
ECO111_RS09635	1843201..1844142	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1833093..1834241	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T24350 WP_000813794.1 NC_013364:1838656-1838832 [Escherichia coli O111:H- str. 11128]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T24350 NC_013364:1838656-1838832 [Escherichia coli O111:H- str. 11128]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT24350 WP_001270286.1 NC_013364:1838878-1839294 [Escherichia coli O111:H- str. 11128]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT24350 NC_013364:1838878-1839294 [Escherichia coli O111:H- str. 11128]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB