Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4344402..4344899 Replicon chromosome
Accession NZ_CP096837
Organism Escherichia coli strain PS10-3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MYF74_RS20440 Protein ID WP_001173343.1
Coordinates 4344402..4344575 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MYF74_RS20445 Protein ID WP_000398619.1
Coordinates 4344603..4344899 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF74_RS20430 (4341072) 4341072..4342529 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
MYF74_RS20435 (4342766) 4342766..4344283 + 1518 WP_001295074.1 lysine--tRNA ligase -
MYF74_RS20440 (4344402) 4344402..4344575 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MYF74_RS20445 (4344603) 4344603..4344899 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MYF74_RS20450 (4345126) 4345126..4345398 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MYF74_RS20455 (4345410) 4345410..4345640 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
MYF74_RS20460 (4345821) 4345821..4347452 + 1632 WP_001216477.1 sensor histidine kinase -
MYF74_RS20465 (4347449) 4347449..4348168 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T243492 WP_001173343.1 NZ_CP096837:c4344575-4344402 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T243492 NZ_OX352940:829069-829914 [Streptococcus suis]
ATGAACAAAGCTAAATTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGTTTAACCTTTAATTCCGTGATGACCTA
TTACTTCCTAGAAGTCATTTTGAAAAAATTAAGTCAAAGTTCTTATTCAAATCACTACATCTTCAAAGGAGGATTCCTAC
TGTCGAATGTCATCGGAGTTGAATCTCGTAGCACTGTTGATATTGACTTTTTATTCCATCAAATAACACTCTCAGAAGAA
ACTGTTAAGCAGCAACTCAAGGAAATTTTAGCAGATTCCGAAGAAGGTATCTCTTTTGTGATTCAATCAATCACAGCTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACCATTTTGTGCCAACTTGAGAATATTAAACAAGTTATTCATTTGG
ATATTGCGACAGGTGATGTTGTAACTCCTCAGCCGATTACATACGACTATAAAGCTATTTTTGATGAAGACAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAGAAACTTCAAACCATCTATTCACGCAACTTCTTGAACAGTAGAAG
CAAAGATTTTTACGATGTTTATATTCTTTCAAAACTGAAGAAAGAAGACATTGACTTCATTCAATTGAAAAATGCCTGTC
AGAGAACATTTTCATATCGCGAAACAGAACTCGATTTTGAAAAGATTATTGAACTACTCGAAAGGTTCAAATCTGACCCT
ATTCAGAATAAACAATGGCAAAATTATTCAAAAAAATATAGCTATACAAAGGGGATTTCACTTGCAGATGTTCTTGATGA
GATGATTCGTTTTATAACAGTTCTCATTTCTATAAATTCTGATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT243492 WP_000398619.1 NZ_CP096837:c4344899-4344603 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT243492 NZ_OX352940:828482-829072 [Streptococcus suis]
ATGTCAAAAAAAGAGATTCTACTCGATTTTATAGAAAAGAACAATGGAATTGTCACAAACAAAGATTGTAAAGCACTAGG
TATTCCAACAATATATCTGACAAGACTAGAGAAAGAAGGCATTATCTTCCGAGTTGAAAAAGGTATTTTCCTAACCCAAA
ACGGAGACTATGATGAATACTACTTCTTTCAATATCGTTTTCCTAAGGCTATTTTCTCTTATATTTCGGCACTGTATCTA
CAACAGTTTACAGATGAAATTCCACAATATTTTGATGTGACAGTTCCTAGAGGCTACCGCTTTAATACTCCTCCAGCCAA
TCTAAATATTCACTTTGTCTCTAAAGAATACAGCGAGCTGGGAATGACTACTGTACCTACTCCTATGGGAAACAACGTAA
GGGTATATGATTTTGAACGTATTATTTGTGATTTTGTAATACATCGAGAAAAGATTGATTCCGAACTCTTTGTCAAGACA
CTTCAATCTTATGGGAACTATCCTAAAAAAAATCTAGCAAAACTCTATGAATATGCGACAAAAATGAATACTTTAGAAAA
AGTTAAACAAACTCTGGAGGTTCTAATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References