Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4010695..4011245 Replicon chromosome
Accession NZ_CP096837
Organism Escherichia coli strain PS10-3

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag MYF74_RS18895 Protein ID WP_001160974.1
Coordinates 4010695..4011009 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag MYF74_RS18900 Protein ID WP_000125566.1
Coordinates 4011012..4011245 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF74_RS18870 (4006370) 4006370..4007656 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
MYF74_RS18875 (4007656) 4007656..4008645 + 990 WP_248748109.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
MYF74_RS18880 (4008642) 4008642..4009463 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
MYF74_RS18885 (4009466) 4009466..4009843 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
MYF74_RS18890 (4009850) 4009850..4010698 + 849 WP_000257184.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
MYF74_RS18895 (4010695) 4010695..4011009 - 315 WP_001160974.1 CcdB family protein Toxin
MYF74_RS18900 (4011012) 4011012..4011245 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
MYF74_RS18905 (4011331) 4011331..4011810 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
MYF74_RS18910 (4012002) 4012002..4013864 - 1863 WP_000377191.1 glutathione-regulated potassium-efflux system protein KefC -
MYF74_RS18915 (4013857) 4013857..4014387 - 531 WP_000600729.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
MYF74_RS18920 (4014498) 4014498..4015829 - 1332 WP_001183198.1 MFS transporter -
MYF74_RS18925 (4015887) 4015887..4016174 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T243490 WP_001160974.1 NZ_CP096837:c4011009-4010695 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T243490 NZ_OX352940:c770478-770209 [Streptococcus suis]
ATGGCTTATAAGCTTGTTCTCAGCGATGATGCTTTGAAGCAGTTGAAAAAAATGGACAGGCATGTGGGGATGATGCTTGC
TAAGGATTTGAAAAAACGTTTGGACGGTTTGGAGAATCCAAGACAATTTGGGAAGGCCTTGGTAGGAGACTATAAGGGGC
TGTGGCGGTATAGAGTGGGGAATTACCGTGTAATTTGTGATATTATTGATAACAAAATGGTCATCCTGGCCCTTGAAATC
GGGCACCGGAAAGAAATCTATAAAAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT243490 WP_000125566.1 NZ_CP096837:c4011245-4011012 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT243490 NZ_OX352940:c770695-770468 [Streptococcus suis]
ATGGGAATTGTATCCTTGCGATTAAACGATGCAGAAGAAGAAATTTTCAGAAGCTATGCTATTCATACAGGAAAAAGTCT
GTCTGAGCTATTCAAAACTGCACTTGCTGAACAGATAGAAGACCAGTTGGACTATGAAATTGGTATCAAGGCTTTGAAGA
AATTCCAAGAAAATCCTGTTACACACTCGATTGATGATGTTCTTGCGGAGTTAGAAGATGGCTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References