243471

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 34749..35271 Replicon plasmid pPM4-5-43k
Accession NZ_CP096836
Organism Escherichia coli strain PM4-5

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag MYF75_RS25755 Protein ID WP_000220560.1
Coordinates 34990..35271 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag MYF75_RS25750 Protein ID WP_000121743.1
Coordinates 34749..35000 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF75_RS25725 (MYF75_25730) 30420..30701 + 282 WP_001185482.1 hypothetical protein -
MYF75_RS25730 (MYF75_25735) 30726..31703 + 978 WP_024245156.1 hypothetical protein -
MYF75_RS25735 (MYF75_25740) 31700..32056 - 357 WP_000456533.1 DNA distortion polypeptide 3 -
MYF75_RS25740 (MYF75_25745) 32193..32639 - 447 Protein_48 hypothetical protein -
MYF75_RS25745 (MYF75_25750) 32679..33515 - 837 WP_001050931.1 RepB family plasmid replication initiator protein -
MYF75_RS25750 (MYF75_25755) 34749..35000 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
MYF75_RS25755 (MYF75_25760) 34990..35271 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MYF75_RS25760 (MYF75_25765) 35346..36043 + 698 WP_103215986.1 IS1-like element IS1A family transposase -
MYF75_RS25765 (MYF75_25770) 36053..36361 + 309 Protein_53 replication initiation protein -
MYF75_RS25770 (MYF75_25775) 37246..38214 + 969 WP_030005799.1 IS5 family transposase -
MYF75_RS25775 (MYF75_25780) 38354..38740 - 387 WP_001039464.1 plasmid stabilization protein StbA -
MYF75_RS25780 (MYF75_25785) 39321..39554 + 234 Protein_56 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid qnrS1 / dfrA14 / sul3 / blaTEM-1B / floR - 1..43439 43439


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T243471 WP_000220560.1 NZ_CP096836:34990-35271 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T243471 NZ_OX352324:c5373740-5373558 [Pseudomonas sp. MM221]
ATGAAATACAGTGAATTTCGCCGATGGCTACGCGGCCAGGGCGCTACGTTCGTGGCAGCCAAGGGCAGTCATTTCAAGGT
TTACCTAGGCAATCGGCAAACGATTTTCCCGGATCATGGCGCGAAGGAAATCAGTGAGGGCCTGAGAAAGAAGATCCTGA
AAGACTTGGGATTGAAAGCATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT243471 WP_000121743.1 NZ_CP096836:34749-35000 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT243471 NZ_OX352324:c5373534-5373112 [Pseudomonas sp. MM221]
ATATTCGAGTACCCGGTGGTCGTTCATCAAGAAGACGGCAGTGTTTGGGTGAGTTGTCCCGATGTACCAGAAATGGCCTG
CGCGGGTGATACTTCGGAGGAGGCGCTGTTCGATGCTGTGGATGCGTTGGAGTCTGCGCTTTCCTTCTATGTGGATCAGA
AGCACGCTATCCCGCTACCTTCAGCGCCTACAGAGGGTCATCCCGTCGTACGTCTGCCAGCCCTGACGGCAGCGAAGGCG
GCGCTGTGGAACACGATGGTGGCACAAAAAATCAGCAAGACCGAGATGGCGCGCAGGCTAGGGGTAAATCGTCCCCAGGT
CGATCGTCTGGTCGATCTGTTGCACCGCTCCAAAATCGAGCAGGTTGAGCATGCGCTGAATGTGTTGGGGCAGCGAATCG
AGCTTTCAGTCGTAGCCGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References