Detailed information of TA system TA243470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 94958..95601
• Replicon: plasmid pPM4-5-103k
• Accession: NZ_CP096835
• Organism: Escherichia coli strain PM4-5

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MYF75_RS25460
• Protein ID: WP_063073410.1
• Coordinates: 95185..95601 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A3Q0N5I0
• Locus tag: MYF75_RS25455
• Protein ID: WP_063073411.1
• Coordinates: 94958..95188 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYF75_RS25415 (90027)	90027..90419	-	393	WP_063073471.1	plasmid partitioning/stability family protein	-
MYF75_RS25420 (90424)	90424..91395	-	972	WP_001103690.1	plasmid segregation protein ParM	-
MYF75_RS25425 (91624)	91624..92268	+	645	WP_000633913.1	division plane positioning ATPase MipZ	-
MYF75_RS25430 (92262)	92262..92537	+	276	WP_050858693.1	hypothetical protein	-
MYF75_RS25435 (92689)	92689..93480	-	792	WP_000016494.1	site-specific integrase	-
MYF75_RS25440 (93477)	93477..93983	-	507	WP_001505085.1	hypothetical protein	-
MYF75_RS25445 (93974)	93974..94678	+	705	WP_001775011.1	IS6-like element IS26 family transposase	-
MYF75_RS25450 (94825)	94825..95001	-	177	Protein_110	hypothetical protein	-
MYF75_RS25455 (94958)	94958..95188	+	231	WP_063073411.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MYF75_RS25460 (95185)	95185..95601	+	417	WP_063073410.1	type II toxin-antitoxin system VapC family toxin	Toxin
MYF75_RS25465 (95675)	95675..97237	+	1563	WP_063073409.1	AAA family ATPase	-
MYF75_RS25470 (97222)	97222..98244	+	1023	WP_063073408.1	hypothetical protein	-
MYF75_RS25475 (98531)	98531..98761	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	-
MYF75_RS25480 (98758)	98758..98853	+	96	Protein_116	VapC toxin family PIN domain ribonuclease	-
MYF75_RS25485 (98912)	98912..99696	-	785	Protein_117	IS21-like element helper ATPase IstB	-
MYF75_RS25490 (99696)	99696..100349	-	654	Protein_118	IS21-like element IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..103920	103920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15167.53 Da
• Isoelectric Point: 7.8882

>T243470 WP_063073410.1 NZ_CP096835:95185-95601 [Escherichia coli]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCTRLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHSIAAGAVLVTNNTREFERVPGLVLEDWIR

Nucleotide

• Length: 417 bp

>T243470 NZ_OX352324:5040865-5041158 [Pseudomonas sp. MM221]
ATGACTGAGACCTTCTCTGAATTCGATCCCGCCGAGTACCTCACTACCCCTGAGGCAATCGCGCTGTTCATGACTGACGC
CCTTGAGACCGGTGATGCCGGCTATGTCGCCAAGGCCGTAGGTGTGGTGGCACGTGCAAAAGGCATGAGCGAACTGGCGA
AAGAGACCGGGCTGTCACGAGAGCAGCTTTACAGGTCTTTCAGCGAGCATGGCAATCCGACCCTGAAGTCTTTTCTGACC
GTGATGAAAGCACTGGGTATAGACATGACGGCGCGCCCCCACCTCGCTGGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8688.84 Da
• Isoelectric Point: 4.6310

>AT243470 WP_063073411.1 NZ_CP096835:94958-95188 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAKLEKADPDFMAEREDVVTDEGRVNL

Nucleotide

• Length: 231 bp

>AT243470 NZ_OX352324:5040570-5040872 [Pseudomonas sp. MM221]
ATGAAGAAAATCGAATCCAGCAGTTTCAGACATTGGGTGACCGGTCTCCGGGACGTTAACGCCAGGGCCCGAATCATTTC
TCGGATCAACCGGTTGATGGAGGGTCTGCCTGGCGACGTATCACCGGTTGGCCAAGGCATTAGTGAACTCAGAATTCACT
ATGGGCCTGGCTACCGGGTTTACTTCCATCAGGTGGGCAGCACATTCGTCATTCTGCTTTGCGGCGGTGAAAAAAGTAGC
CAACAGCGAGACATCAAGACAGCCCACCAGATTCTGCGTTCGTGGAGGATACAAAATGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A3Q0N5I0