Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 33311..33870 | Replicon | plasmid pPM4-5-103k |
| Accession | NZ_CP096835 | ||
| Organism | Escherichia coli strain PM4-5 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | A0A1V2T4U7 |
| Locus tag | MYF75_RS25045 | Protein ID | WP_063073486.1 |
| Coordinates | 33311..33586 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | A0A1V2T5E1 |
| Locus tag | MYF75_RS25050 | Protein ID | WP_062894229.1 |
| Coordinates | 33586..33870 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MYF75_RS25020 (29109) | 29109..30245 | - | 1137 | WP_033804283.1 | porin | - |
| MYF75_RS25025 (30799) | 30799..32027 | + | 1229 | WP_167654659.1 | IS3 family transposase | - |
| MYF75_RS25030 (32096) | 32096..32285 | + | 190 | Protein_26 | IS66 family insertion sequence element accessory protein TnpB | - |
| MYF75_RS25035 (32288) | 32288..32476 | - | 189 | WP_063073487.1 | DUF1187 family protein | - |
| MYF75_RS25040 (32556) | 32556..33044 | + | 489 | Protein_28 | transposase | - |
| MYF75_RS25045 (33311) | 33311..33586 | - | 276 | WP_063073486.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| MYF75_RS25050 (33586) | 33586..33870 | - | 285 | WP_062894229.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| MYF75_RS25055 (34781) | 34781..35638 | - | 858 | WP_019842754.1 | incFII family plasmid replication initiator RepA | - |
| MYF75_RS25060 (35631) | 35631..36113 | - | 483 | WP_074149688.1 | hypothetical protein | - |
| MYF75_RS25065 (36106) | 36106..36153 | - | 48 | WP_229471593.1 | hypothetical protein | - |
| MYF75_RS25070 (36144) | 36144..36395 | + | 252 | WP_223195197.1 | replication protein RepA | - |
| MYF75_RS25075 (36412) | 36412..36669 | - | 258 | WP_000083845.1 | replication regulatory protein RepA | - |
| MYF75_RS25080 (36932) | 36932..37078 | - | 147 | Protein_36 | Hok/Gef family protein | - |
| - (37122) | 37122..37183 | + | 62 | NuclAT_2 | - | - |
| - (37122) | 37122..37183 | + | 62 | NuclAT_2 | - | - |
| - (37122) | 37122..37183 | + | 62 | NuclAT_2 | - | - |
| - (37122) | 37122..37183 | + | 62 | NuclAT_2 | - | - |
| MYF75_RS25085 (37766) | 37766..37978 | - | 213 | WP_032084501.1 | hypothetical protein | - |
| MYF75_RS25090 (38112) | 38112..38672 | - | 561 | WP_063073483.1 | fertility inhibition protein FinO | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..103920 | 103920 | |
| - | inside | IScluster/Tn | - | - | 5581..33044 | 27463 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10724.40 Da Isoelectric Point: 9.8136
>T243466 WP_063073486.1 NZ_CP096835:c33586-33311 [Escherichia coli]
MELKWTSKALSDLARLYDFLALASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIYAGEYEIRYELTGQTIY
VLRLWHTRENR
MELKWTSKALSDLARLYDFLALASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIYAGEYEIRYELTGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T243466 NZ_OX352324:c3568120-3567872 [Pseudomonas sp. MM221]
ATGTCCAAGCAAGAAAAGCTGCTCGCGAAGCTGCTGAACAAGCAAGCAGGATTCACTTGGCCGGAGCTGGTTACGCTGCT
GGGTGGGCTTGGATACCGCCAGATCGAGGGTGACGGCAGCCGGGTAAAATTCGACAACGGCCACCCGCAGGCGATGATCA
ACCTGCACAAGCCGCACCCCGGCAATGAGCTGAAGGCCTACGTTAAACGCCAGGTGATAGAACACCTGAAGGCAGGAGGA
TTGATCTGA
ATGTCCAAGCAAGAAAAGCTGCTCGCGAAGCTGCTGAACAAGCAAGCAGGATTCACTTGGCCGGAGCTGGTTACGCTGCT
GGGTGGGCTTGGATACCGCCAGATCGAGGGTGACGGCAGCCGGGTAAAATTCGACAACGGCCACCCGCAGGCGATGATCA
ACCTGCACAAGCCGCACCCCGGCAATGAGCTGAAGGCCTACGTTAAACGCCAGGTGATAGAACACCTGAAGGCAGGAGGA
TTGATCTGA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10486.95 Da Isoelectric Point: 5.6558
>AT243466 WP_062894229.1 NZ_CP096835:c33870-33586 [Escherichia coli]
MQMKNNTAQVTKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLGTDNPLPVPR
MQMKNNTAQVTKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLGTDNPLPVPR
Download Length: 285 bp
>AT243466 NZ_OX352324:c3567872-3567531 [Pseudomonas sp. MM221]
ATGAGCACCATGTTGCAGCACCGAGGCTACTACGGCTCCATTGAGGCCAGCCCCGAAGACAATTGCCTGTTCGGCAAGCT
CCAGTTCATCCGCGCCCTTGTCAGCTATGAGGGCGAGACGGTTGCCGAGCTGACCCAGGCGTTCCGCGATGCAGTGGACG
ACTACCTGGATACCTGCGCATCACTCGGGCAGGAGCCGGAGATTCCGTGCAAGGGGTCCTTCAACGTGAGGGTAGGGCAC
GACCTGCACCTGGCAGCCAGCGTGGCGGCTACCCAGCAAAACATTTCTTTGAATGACCTCACCCGTAAAGCGCTCAGCGA
ATACCTGGGCCATCACGCATAG
ATGAGCACCATGTTGCAGCACCGAGGCTACTACGGCTCCATTGAGGCCAGCCCCGAAGACAATTGCCTGTTCGGCAAGCT
CCAGTTCATCCGCGCCCTTGTCAGCTATGAGGGCGAGACGGTTGCCGAGCTGACCCAGGCGTTCCGCGATGCAGTGGACG
ACTACCTGGATACCTGCGCATCACTCGGGCAGGAGCCGGAGATTCCGTGCAAGGGGTCCTTCAACGTGAGGGTAGGGCAC
GACCTGCACCTGGCAGCCAGCGTGGCGGCTACCCAGCAAAACATTTCTTTGAATGACCTCACCCGTAAAGCGCTCAGCGA
ATACCTGGGCCATCACGCATAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1V2T4U7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1V2T5E1 |