243465

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 15663..16188 Replicon plasmid pPM4-5-103k
Accession NZ_CP096835
Organism Escherichia coli strain PM4-5

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MYF75_RS24965 Protein ID WP_001159871.1
Coordinates 15663..15968 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag MYF75_RS24970 Protein ID WP_000813634.1
Coordinates 15970..16188 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF75_RS24935 (11576) 11576..12556 + 981 WP_000019440.1 IS5-like element ISKpn26 family transposase -
MYF75_RS24940 (12822) 12822..13139 - 318 Protein_8 integrase core domain-containing protein -
MYF75_RS24945 (13125) 13125..13289 + 165 WP_225390958.1 hypothetical protein -
MYF75_RS24950 (13445) 13445..13945 + 501 Protein_10 IS21-like element IS100 family transposase -
MYF75_RS24955 (13942) 13942..14637 + 696 WP_074440817.1 AAA family ATPase -
MYF75_RS24960 (14856) 14856..15662 - 807 WP_020219156.1 site-specific integrase -
MYF75_RS24965 (15663) 15663..15968 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MYF75_RS24970 (15970) 15970..16188 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MYF75_RS24975 (17004) 17004..17294 - 291 WP_032248853.1 hypothetical protein -
MYF75_RS24980 (17593) 17593..18755 - 1163 Protein_16 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..103920 103920
- inside IScluster/Tn - - 5581..33044 27463


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T243465 WP_001159871.1 NZ_CP096835:c15968-15663 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T243465 NZ_OX352324:c1466857-1466579 [Pseudomonas sp. MM221]
ATGATTCGAAGCTTTAGCTGTGCCGAAACGGAAGCGCTCTTTTGCACCGGAAAGACACGGCGTTGGTCAGATATCAAGTC
AGTCGCGGAGCGCAAACTGGCCATGCTCGATGCCGCAACAGCGTTGCGAGACCTTCGGTCGCCACCTGGCAATCGCCTCG
AGTCACTGAGCGGCAACCGCGCTGGTCAACACAGTATTCGTGTGAATGACCAATGGCGCGTGTGCTTCACATGGACAGAG
CACGGGCCAGTCAACGTTAAAATTGTCGATTACCACTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT243465 WP_000813634.1 NZ_CP096835:c16188-15970 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT243465 NZ_OX352324:c1466570-1466271 [Pseudomonas sp. MM221]
ATGCTCAAGAACGGTATGCGTCCAATCCATCCGGGCGAAATCCTGCGTGAGGACTTCCAGAAGGAAATGGGCTTTAGCGC
CGCGGCACTGGCCAGGGCGTTGGGCGTTGCAACACCAACCGTCAACAATATCCTGCGCGAAAGGGGCGGTATCTCCGCCG
ACATGGCACTGCGCTTGTCCATCTGCCTGGACACCACACCCGAGTTCTGGCTCAACCTGCAGACGGCCTTCGATTTGCGC
ACTGCCGAACAGCAACACGGTGATGAGATCATCGAGTCGGTCCAGCGCCTGGTCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References