Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 15663..16188 Replicon plasmid pPM4-5-103k
Accession NZ_CP096835
Organism Escherichia coli strain PM4-5

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MYF75_RS24965 Protein ID WP_001159871.1
Coordinates 15663..15968 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag MYF75_RS24970 Protein ID WP_000813634.1
Coordinates 15970..16188 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF75_RS24935 (11576) 11576..12556 + 981 WP_000019440.1 IS5-like element ISKpn26 family transposase -
MYF75_RS24940 (12822) 12822..13139 - 318 Protein_8 integrase core domain-containing protein -
MYF75_RS24945 (13125) 13125..13289 + 165 WP_225390958.1 hypothetical protein -
MYF75_RS24950 (13445) 13445..13945 + 501 Protein_10 IS21-like element IS100 family transposase -
MYF75_RS24955 (13942) 13942..14637 + 696 WP_074440817.1 AAA family ATPase -
MYF75_RS24960 (14856) 14856..15662 - 807 WP_020219156.1 site-specific integrase -
MYF75_RS24965 (15663) 15663..15968 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MYF75_RS24970 (15970) 15970..16188 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MYF75_RS24975 (17004) 17004..17294 - 291 WP_032248853.1 hypothetical protein -
MYF75_RS24980 (17593) 17593..18755 - 1163 Protein_16 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..103920 103920
- inside IScluster/Tn - - 5581..33044 27463


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T243465 WP_001159871.1 NZ_CP096835:c15968-15663 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T243465 NZ_OX352324:c1466857-1466579 [Pseudomonas sp. MM221]
ATGATTCGAAGCTTTAGCTGTGCCGAAACGGAAGCGCTCTTTTGCACCGGAAAGACACGGCGTTGGTCAGATATCAAGTC
AGTCGCGGAGCGCAAACTGGCCATGCTCGATGCCGCAACAGCGTTGCGAGACCTTCGGTCGCCACCTGGCAATCGCCTCG
AGTCACTGAGCGGCAACCGCGCTGGTCAACACAGTATTCGTGTGAATGACCAATGGCGCGTGTGCTTCACATGGACAGAG
CACGGGCCAGTCAACGTTAAAATTGTCGATTACCACTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT243465 WP_000813634.1 NZ_CP096835:c16188-15970 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT243465 NZ_OX352324:c1466570-1466271 [Pseudomonas sp. MM221]
ATGCTCAAGAACGGTATGCGTCCAATCCATCCGGGCGAAATCCTGCGTGAGGACTTCCAGAAGGAAATGGGCTTTAGCGC
CGCGGCACTGGCCAGGGCGTTGGGCGTTGCAACACCAACCGTCAACAATATCCTGCGCGAAAGGGGCGGTATCTCCGCCG
ACATGGCACTGCGCTTGTCCATCTGCCTGGACACCACACCCGAGTTCTGGCTCAACCTGCAGACGGCCTTCGATTTGCGC
ACTGCCGAACAGCAACACGGTGATGAGATCATCGAGTCGGTCCAGCGCCTGGTCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References