Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4089080..4089705 Replicon chromosome
Accession NZ_CP096834
Organism Escherichia coli strain PM4-5

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag MYF75_RS21380 Protein ID WP_000911329.1
Coordinates 4089307..4089705 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag MYF75_RS21375 Protein ID WP_000450524.1
Coordinates 4089080..4089307 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF75_RS21350 (4084883) 4084883..4085353 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
MYF75_RS21355 (4085353) 4085353..4085925 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
MYF75_RS21360 (4086071) 4086071..4086949 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
MYF75_RS21365 (4086966) 4086966..4088000 + 1035 WP_113441149.1 outer membrane protein assembly factor BamC -
MYF75_RS21370 (4088213) 4088213..4088926 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
MYF75_RS21375 (4089080) 4089080..4089307 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
MYF75_RS21380 (4089307) 4089307..4089705 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
MYF75_RS21385 (4089852) 4089852..4090715 + 864 WP_001267507.1 neutral zinc metallopeptidase -
MYF75_RS21390 (4090730) 4090730..4092745 + 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
MYF75_RS21395 (4092819) 4092819..4093517 + 699 WP_000679812.1 esterase -
MYF75_RS21400 (4093598) 4093598..4093798 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T243463 WP_000911329.1 NZ_CP096834:4089307-4089705 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T243463 NZ_OX352324:c807037-806660 [Pseudomonas sp. MM221]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT243463 WP_000450524.1 NZ_CP096834:4089080-4089307 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT243463 NZ_OX352324:c806679-806344 [Pseudomonas sp. MM221]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References