Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3792254..3792837 Replicon chromosome
Accession NZ_CP096834
Organism Escherichia coli strain PM4-5

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag MYF75_RS19935 Protein ID WP_000254745.1
Coordinates 3792502..3792837 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag MYF75_RS19930 Protein ID WP_000581937.1
Coordinates 3792254..3792502 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF75_RS19920 (3788593) 3788593..3789894 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
MYF75_RS19925 (3789942) 3789942..3792176 + 2235 WP_000226815.1 GTP pyrophosphokinase -
MYF75_RS19930 (3792254) 3792254..3792502 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MYF75_RS19935 (3792502) 3792502..3792837 + 336 WP_000254745.1 endoribonuclease MazF Toxin
MYF75_RS19940 (3792908) 3792908..3793699 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
MYF75_RS19945 (3793927) 3793927..3795564 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
MYF75_RS19950 (3795652) 3795652..3796950 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T243460 WP_000254745.1 NZ_CP096834:3792502-3792837 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T243460 NZ_OX352322:c5374938-5374756 [Pseudomonas sp. MM223]
ATGAAATACAGTGAATTTCGCCGATGGCTACGCGGCCAGGGCGCTACGTTCGTGGCAGCCAAGGGCAGTCATTTCAAGGT
TTACCTAGGCAATCGGCAAACGATTTTCCCGGATCATGGCGCGAAGGAAATCAGTGAGGGCCTGAGAAAGAAGATCCTGA
AAGACTTGGGATTGAAAGCATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT243460 WP_000581937.1 NZ_CP096834:3792254-3792502 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT243460 NZ_OX352322:c5374732-5374310 [Pseudomonas sp. MM223]
ATATTCGAGTACCCGGTGGTCGTTCATCAAGAAGACGGCAGTGTTTGGGTGAGTTGTCCCGATGTACCAGAAATGGCCTG
CGCGGGTGATACTTCGGAGGAGGCGCTGTTCGATGCTGTGGATGCGTTGGAGTCTGCGCTTTCCTTCTATGTGGATCAGA
AGCACGCTATCCCGCTACCTTCAGCGCCTACAGAGGGTCATCCCGTCGTACGTCTGCCAGCCCTGACGGCAGCGAAGGCG
GCGCTGTGGAACACGATGGTGGCACAAAAAATCAGCAAGACCGAGATGGCGCGCAGGCTAGGGGTAAATCGTCCCCAGGT
CGATCGTCTGGTCGATCTGTTGCACCGCTCCAAAATCGAGCAGGTTGAGCATGCGCTGAATGTGTTGGGGCAGCGAATCG
AGCTTTCAGTCGTAGCCGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References