Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3632887..3633541 Replicon chromosome
Accession NZ_CP096834
Organism Escherichia coli strain PM4-5

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag MYF75_RS19180 Protein ID WP_000244777.1
Coordinates 3633134..3633541 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MYF75_RS19175 Protein ID WP_000354046.1
Coordinates 3632887..3633153 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF75_RS19150 (3628175) 3628175..3628918 + 744 WP_000951948.1 SDR family oxidoreductase -
MYF75_RS19155 (3628975) 3628975..3630408 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
MYF75_RS19160 (3630453) 3630453..3630764 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
MYF75_RS19165 (3630928) 3630928..3631587 + 660 WP_000250274.1 hemolysin III family protein -
MYF75_RS19170 (3631664) 3631664..3632644 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MYF75_RS19175 (3632887) 3632887..3633153 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MYF75_RS19180 (3633134) 3633134..3633541 + 408 WP_000244777.1 protein YgfX Toxin
MYF75_RS19185 (3633581) 3633581..3634102 - 522 WP_001055874.1 flavodoxin FldB -
MYF75_RS19190 (3634214) 3634214..3635110 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MYF75_RS19195 (3635135) 3635135..3635845 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MYF75_RS19200 (3635851) 3635851..3637584 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T243459 WP_000244777.1 NZ_CP096834:3633134-3633541 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T243459 NZ_OX352322:5041980-5042273 [Pseudomonas sp. MM223]
ATGACTGAGACCTTCTCTGAATTCGATCCCGCCGAGTACCTCACTACCCCTGAGGCAATCGCGCTGTTCATGACTGACGC
CCTTGAGACCGGTGATGCCGGCTATGTCGCCAAGGCCGTAGGTGTGGTGGCACGTGCAAAAGGCATGAGCGAACTGGCGA
AAGAGACCGGGCTGTCACGAGAGCAGCTTTACAGGTCTTTCAGCGAGCATGGCAATCCGACCCTGAAGTCTTTTCTGACC
GTGATGAAAGCACTGGGTATAGACATGACGGCGCGCCCCCACCTCGCTGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT243459 WP_000354046.1 NZ_CP096834:3632887-3633153 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT243459 NZ_OX352322:5041685-5041987 [Pseudomonas sp. MM223]
ATGAAGAAAATCGAATCCAGCAGTTTCAGACATTGGGTGACCGGTCTCCGGGACGTTAACGCCAGGGCCCGAATCATTTC
TCGGATCAACCGGTTGATGGAGGGTCTGCCTGGCGACGTATCACCGGTTGGCCAAGGCATTAGTGAACTCAGAATTCACT
ATGGGCCTGGCTACCGGGTTTACTTCCATCAGGTGGGCAGCACATTCGTCATTCTGCTTTGCGGCGGTGAAAAAAGTAGC
CAACAGCGAGACATCAAGACAGCCCACCAGATTCTGCGTTCGTGGAGGATACAAAATGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References