Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3059140..3059940 Replicon chromosome
Accession NZ_CP096834
Organism Escherichia coli strain PM4-5

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag MYF75_RS16290 Protein ID WP_000342449.1
Coordinates 3059413..3059940 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag MYF75_RS16285 Protein ID WP_001277108.1
Coordinates 3059140..3059406 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF75_RS16265 (3054798) 3054798..3055466 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
MYF75_RS16270 (3055459) 3055459..3056517 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
MYF75_RS16275 (3056762) 3056762..3057616 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
MYF75_RS16280 (3057887) 3057887..3058990 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
MYF75_RS16285 (3059140) 3059140..3059406 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
MYF75_RS16290 (3059413) 3059413..3059940 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
MYF75_RS16295 (3059937) 3059937..3060320 - 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
MYF75_RS16300 (3060744) 3060744..3061853 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
MYF75_RS16305 (3061901) 3061901..3062827 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MYF75_RS16310 (3062824) 3062824..3064101 + 1278 WP_047659341.1 branched chain amino acid ABC transporter permease LivM -
MYF75_RS16315 (3064098) 3064098..3064865 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T243457 WP_000342449.1 NZ_CP096834:3059413-3059940 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T243457 NZ_OX352322:c4742381-4742085 [Pseudomonas sp. MM223]
ATGGAAAAGAGAACGCCCCACTGTCCGCTGGAGCGGGTGAGAGCCTTGGCTGCTGCAAGGTGCATCAGGCCTACAGGCGC
TGCATTGAGAGGCGCCAAAGCGCTGGGAATGGATTATCCGGGCATGCTTGAAGTGATCTCCAGTTTGGAGCGCACTGATT
TTTACAAGAGCATGACCAGCCATATGGATCACCGGGTTTGGCAGGACGTCTATCGTCCGCTTACAGCAATTGGGTACGTT
TATCTGAAACTGTCCGTGGTAGACGACGTGCTCATCGTGTCTTTCAAGGGGTTGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT243457 WP_001277108.1 NZ_CP096834:3059140-3059406 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT243457 NZ_OX352322:c4742082-4741681 [Pseudomonas sp. MM223]
ATGAAATGTCCAATCTGCGGCGGTGCGGAGCTTGCACCTGATGTTCAGGGCATGCCCTTTAGCTATAAAGGAGAGGCGAC
CGTAATCCCTGACGTAAGTGGCGATTATTGTTCCGCATGTGGTGAATGTGTACTTAGCCACGATGAAGCCATGCGTGTCA
GTCACCTGATGACGGCATTCGAGCGCCAAGTCAACGCAACTGTTGTGGATCCTTCCTTCATTGCTTCAATGCGCCGGAAG
TTCGACCTCGACCAGCGTGAAGCGGGGGAAATTTTCGGTGGTGGCGTCAATGCGTTCTCTCGTTATGAAAACGGCAAGAC
CACGCCGCCGGTGGCGTTGGTGAAACTGCTCAAGCTGCTGGATCGCCATCCAGAGCTTTTCGAGGAAGTACGTACTGCCT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References