243456

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3037850..3038436 Replicon chromosome
Accession NZ_CP096834
Organism Escherichia coli strain PM4-5

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag MYF75_RS16165 Protein ID WP_042014162.1
Coordinates 3037850..3038080 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag MYF75_RS16170 Protein ID WP_000593555.1
Coordinates 3038077..3038436 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF75_RS16155 (3033887) 3033887..3036622 + 2736 WP_113441165.1 ribosome-associated ATPase/putative transporter RbbA -
MYF75_RS16160 (3036622) 3036622..3037746 + 1125 WP_001314210.1 ABC transporter permease -
MYF75_RS16165 (3037850) 3037850..3038080 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
MYF75_RS16170 (3038077) 3038077..3038436 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MYF75_RS16175 (3038556) 3038556..3038957 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
MYF75_RS16180 (3038963) 3038963..3039769 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
MYF75_RS16185 (3039766) 3039766..3040530 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
MYF75_RS16190 (3040530) 3040530..3041363 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
MYF75_RS16195 (3041360) 3041360..3042304 - 945 WP_032254808.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T243456 WP_042014162.1 NZ_CP096834:3037850-3038080 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T243456 NZ_OX352322:c4197367-4197086 [Pseudomonas sp. MM223]
ATGACCTATAAGCTTGAATTCCTGCCTTCGGCGCTGAAGGAGTGGGGCAAACTGGGCCACACCGTGCGCGAACAGATCAA
GAAAAAACTTGGCGAACGTTTGCAATCGCCCAAGGTACAGGCCGATGCACTCCGCGACCTGGCGAACCACTATAAAATCA
AGCTCAAGGCCTCCGGATATCGCCTGGTCTATCGGGTCGAGGACGAGCGTATCGTTGTGGTGGTGGTATCGGTTGGAAAG
CGCGAGCGCAGCGAGGTCTATCGAGCCGCTCAGAAGCGCTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT243456 WP_000593555.1 NZ_CP096834:3038077-3038436 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT243456 NZ_OX352322:c4197602-4197357 [Pseudomonas sp. MM223]
ATGCAGAACATCTTTGCTGACGTGGCCGTAAGCGTGTCGGAACTGAAAAAGAACCCATCTGCTGTTATGACCGGCGCTGC
GGGCATGCCCGTCGCCGTGCTTAACCACAATCGCGTGATGGGGTACATGGTACCTGCCGAGCTGTATGAGGCCATGATGG
AGCGCCTGGAAGATCTCGAACTGAGCGAATTGGCCAAGGCCAGGCTGGGTGAAAAAGGCGTCCCGGTGTCTCTGGATGAC
CTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References