Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2290327..2290824 Replicon chromosome
Accession NZ_CP096834
Organism Escherichia coli strain PM4-5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MYF75_RS12760 Protein ID WP_001173343.1
Coordinates 2290327..2290500 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MYF75_RS12765 Protein ID WP_000398619.1
Coordinates 2290528..2290824 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF75_RS12750 (2286997) 2286997..2288454 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
MYF75_RS12755 (2288691) 2288691..2290208 + 1518 WP_001295087.1 lysine--tRNA ligase -
MYF75_RS12760 (2290327) 2290327..2290500 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MYF75_RS12765 (2290528) 2290528..2290824 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MYF75_RS12770 (2291051) 2291051..2291323 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MYF75_RS12775 (2291335) 2291335..2291565 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
MYF75_RS12780 (2291746) 2291746..2293377 + 1632 WP_113441013.1 sensor histidine kinase -
MYF75_RS12785 (2293374) 2293374..2294093 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T243454 WP_001173343.1 NZ_CP096834:c2290500-2290327 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T243454 NZ_OX352322:c1467213-1466935 [Pseudomonas sp. MM223]
ATGATTCGAAGCTTTAGCTGTGCCGAAACGGAAGCGCTCTTTTGCACCGGAAAGACACGGCGTTGGTCAGATATCAAGTC
AGTCGCGGAGCGCAAACTGGCCATGCTCGATGCCGCAACAGCGTTGCGAGACCTTCGGTCGCCACCTGGCAATCGCCTCG
AGTCACTGAGCGGCAACCGCGCTGGTCAACACAGTATTCGTGTGAATGACCAATGGCGCGTGTGCTTCACATGGACAGAG
CACGGGCCAGTCAACGTTAAAATTGTCGATTACCACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT243454 WP_000398619.1 NZ_CP096834:c2290824-2290528 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT243454 NZ_OX352322:c1466926-1466627 [Pseudomonas sp. MM223]
ATGCTCAAGAACGGTATGCGTCCAATCCATCCGGGCGAAATCCTGCGTGAGGACTTCCAGAAGGAAATGGGCTTTAGCGC
CGCGGCACTGGCCAGGGCGTTGGGCGTTGCAACACCAACCGTCAACAATATCCTGCGCGAAAGGGGCGGTATCTCCGCCG
ACATGGCACTGCGCTTGTCCATCTGCCTGGACACCACACCCGAGTTCTGGCTCAACCTGCAGACGGCCTTCGATTTGCGC
ACTGCCGAACAGCAACACGGTGATGAGATCATCGAGTCGGTCCAGCGCCTGGTCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References