Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2186598..2187193 Replicon chromosome
Accession NZ_CP096834
Organism Escherichia coli strain PM4-5

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag MYF75_RS12240 Protein ID WP_000239579.1
Coordinates 2186598..2186948 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag MYF75_RS12245 Protein ID WP_001223208.1
Coordinates 2186942..2187193 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF75_RS12220 (2182044) 2182044..2183066 - 1023 WP_001296689.1 ABC transporter permease -
MYF75_RS12225 (2183080) 2183080..2184582 - 1503 WP_000205813.1 sugar ABC transporter ATP-binding protein -
MYF75_RS12230 (2184722) 2184722..2185678 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
MYF75_RS12235 (2185988) 2185988..2186518 + 531 WP_000055072.1 inorganic diphosphatase -
MYF75_RS12240 (2186598) 2186598..2186948 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
MYF75_RS12245 (2186942) 2186942..2187193 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
MYF75_RS12250 (2187405) 2187405..2187746 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
MYF75_RS12255 (2187749) 2187749..2191528 - 3780 WP_000060927.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T243453 WP_000239579.1 NZ_CP096834:c2186948-2186598 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T243453 NZ_OX352322:c807225-806848 [Pseudomonas sp. MM223]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT243453 WP_001223208.1 NZ_CP096834:c2187193-2186942 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT243453 NZ_OX352322:c806867-806532 [Pseudomonas sp. MM223]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References