Detailed information of TA system TA243452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 2135738..2137073
• Replicon: chromosome
• Accession: NZ_CP096834
• Organism: Escherichia coli strain PM4-5

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: F4VMR9
• Locus tag: MYF75_RS12010
• Protein ID: WP_000342497.1
• Coordinates: 2135738..2136685 (-)
• Length: 316 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: E0J2C6
• Locus tag: MYF75_RS12015
• Protein ID: WP_000152741.1
• Coordinates: 2136678..2137073 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYF75_RS11980 (2131591)	2131591..2132307	+	717	WP_001295734.1	N-acetylneuraminic acid outer membrane channel NanC	-
MYF75_RS11985 (2132327)	2132327..2133433	+	1107	WP_001298927.1	N-acetylneuraminate epimerase	-
MYF75_RS11990 (2133498)	2133498..2134478	+	981	WP_000991446.1	sialate O-acetylesterase	-
MYF75_RS11995 (2134486)	2134486..2135136	-	651	WP_001037963.1	HNH endonuclease	-
MYF75_RS12000 (2135273)	2135273..2135416	+	144	Protein_2039	HNH endonuclease	-
MYF75_RS12005 (2135515)	2135515..2135700	+	186	WP_000066585.1	hypothetical protein	-
MYF75_RS12010 (2135738)	2135738..2136685	-	948	WP_000342497.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
MYF75_RS12015 (2136678)	2136678..2137073	-	396	WP_000152741.1	DUF6088 family protein	Antitoxin
MYF75_RS12020 (2137143)	2137143..2137976	-	834	WP_113441173.1	DUF4942 domain-containing protein	-
MYF75_RS12025 (2138282)	2138282..2139838	+	1557	WP_088568604.1	type I restriction-modification system subunit M	-
MYF75_RS12030 (2139835)	2139835..2141040	+	1206	WP_074598901.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-303)

Antitoxin

• Protein of unknown function DUF6088 (7-108)

Sequences

Toxin

Protein

• Length: 316 a.a.
• Molecular weight: 35588.79 Da
• Isoelectric Point: 4.9737

>T243452 WP_000342497.1 NZ_CP096834:c2136685-2135738 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Nucleotide

• Length: 948 bp

>T243452 NZ_OX352320:c4503859-4503581 [Rheinheimera sp. MM224]
ATGATTAAGAACTTTAAACATAAAGGTCTTGAGCGCTTCTATAAAACAGGCAGTACGGCTGGCATAAAGGCCAAGCATCA
GAATCGCCTGAGACTTATCCTGTCAAACCTTGATCAGGCGGAAGCCATAGATGATATGGATCTGCCTGGCCTTCGGCTGC
ATGAACTTTCGGGTGACCGGAAAGGTATTTGGTCTGTCACCGTGAATGGAAATTGGCGCGTCACATTCAGATTTGTTGGC
CGGGATGCTGAAATTGTTAACTACGAGGATTATCACTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.93 Da
• Isoelectric Point: 11.6915

>AT243452 WP_000152741.1 NZ_CP096834:c2137073-2136678 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRELVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Nucleotide

• Length: 396 bp

>AT243452 NZ_OX352320:c4503581-4503285 [Rheinheimera sp. MM224]
ATGAGTATGCATAACCCGGCTCACCCTGGTGAGATTCTGAAAGAGCTGATTATTGAGCCTTTGGCTTTAACTGTGACTGA
TGTTTCGGCGCATTTAAATATCAGCCGTAAAACCTTATCCAAAGTACTGAATGCCAGAGGCGCTATTACACCGGAAATGG
CACTGCGTCTGGAACTGGCATTTAGCAAACCTTCGGCTGAGCATTGGCTTCGGTTACAAAACGCCTACGATCTGTGGCTG
GTGCGTGAGCAACAAGCACAATTACAGGTGTTGCCTTATCAGTTGGAATTTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1L5BAQ6

Antitoxin

• AlphaFold DB: A0A1L4ZXD4