Detailed information of TA system TA024345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1441818..1442289
• Replicon: chromosome
• Accession: NC_013364
• Organism: Escherichia coli O111:H- str. 11128

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: ECO111_RS07475
• Protein ID: WP_001303511.1
• Coordinates: 1441818..1442096 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: ECO111_RS07480
• Protein ID: WP_001302048.1
• Coordinates: 1442098..1442289 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO111_RS07445	1437125..1437394	-	270	WP_000003742.1	excisionase	-
ECO111_RS07450	1437456..1439921	-	2466	WP_000048442.1	exonuclease	-
ECO111_RS07455	1440014..1440205	-	192	WP_001090200.1	DUF1482 family protein	-
ECO111_RS30950	1440202..1440390	-	189	WP_000449192.1	cell division inhibition protein DicB	-
ECO111_RS07460	1440874..1441092	-	219	WP_001171930.1	hypothetical protein	-
ECO111_RS07465	1441133..1441522	-	390	WP_001447517.1	hypothetical protein	-
ECO111_RS07475	1441818..1442096	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECO111_RS07480	1442098..1442289	-	192	WP_001302048.1	hypothetical protein	Antitoxin
ECO111_RS30960	1442310..1442681	-	372	WP_001169687.1	hypothetical protein	-
ECO111_RS07490	1442779..1443081	+	303	WP_001444689.1	transcriptional regulator	-
ECO111_RS07495	1443078..1443503	+	426	WP_000693943.1	Rha family transcriptional regulator	-
ECO111_RS07500	1443526..1444488	+	963	WP_000095671.1	helix-turn-helix domain-containing protein	-
ECO111_RS07505	1444529..1444951	+	423	WP_001151120.1	DUF977 family protein	-
ECO111_RS07510	1444948..1445202	+	255	WP_000004322.1	hypothetical protein	-
ECO111_RS07515	1445195..1445506	+	312	WP_001002672.1	hypothetical protein	-
ECO111_RS07520	1445812..1446390	+	579	WP_001204666.1	sce7726 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espX7/nleL / espN / espK	1436038..1493197	57159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T24345 WP_001303511.1 NC_013364:c1442096-1441818 [Escherichia coli O111:H- str. 11128]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T24345 NC_013364:c1442096-1441818 [Escherichia coli O111:H- str. 11128]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT24345 WP_001302048.1 NC_013364:c1442289-1442098 [Escherichia coli O111:H- str. 11128]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT24345 NC_013364:c1442289-1442098 [Escherichia coli O111:H- str. 11128]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0