Detailed information of TA system TA243448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1568760..1569597
• Replicon: chromosome
• Accession: NZ_CP096834
• Organism: Escherichia coli strain PM4-5

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: MYF75_RS09385
• Protein ID: WP_000227784.1
• Coordinates: 1569055..1569597 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: MYF75_RS09380
• Protein ID: WP_001297137.1
• Coordinates: 1568760..1569071 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYF75_RS09355 (1563780)	1563780..1564727	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
MYF75_RS09360 (1564749)	1564749..1566740	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
MYF75_RS09365 (1566730)	1566730..1567344	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
MYF75_RS09370 (1567344)	1567344..1567673	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
MYF75_RS09375 (1567685)	1567685..1568575	+	891	WP_000971336.1	heme o synthase	-
MYF75_RS09380 (1568760)	1568760..1569071	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
MYF75_RS09385 (1569055)	1569055..1569597	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
MYF75_RS09390 (1569653)	1569653..1570450	-	798	WP_226397351.1	tetratricopeptide repeat protein	-
MYF75_RS09395 (1570554)	1570554..1571716	+	1163	WP_112026978.1	IS3-like element IS3 family transposase	-
MYF75_RS09400 (1571748)	1571748..1571909	-	162	Protein_1529	sel1 repeat family protein	-
MYF75_RS09405 (1572257)	1572257..1573621	+	1365	WP_001000978.1	MFS transporter	-
MYF75_RS09410 (1573749)	1573749..1574240	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T243448 WP_000227784.1 NZ_CP096834:1569055-1569597 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T243448 NZ_OX352320:548723-549220 [Rheinheimera sp. MM224]
ATAATGCAAACTGTATTACTGGATAAATCGAAGCACGACAGAAATCGATTTAACTGTGGTATTGATGCGTTAAACAACTA
CTTAAAAGTCATGGCCAGTCAGCAAAGTAAAAAAGACAATACCCGTACTTTTGTGCTTGAAGATAAAGCTAACCCACAAC
ACATTGTTGGTTTTTATACTCTGACCATGACTCCGATAGATATACAAAGTCTTCCGGCATCACTGCAGAAAAAACACCAG
TCTTCAACCTCGGGTGGGCTGATCGCACGACTGGCAGTGGATGAAAGATACAAAGGTCAGAAAACAGGCGAGTGGATGTT
GATTGACGCTGTTAAAAAACTACTGCAAGCCAGTGATGCAGTAGGTTTCCCTATTGTTATTGTGGATGCAAAAGACGGCG
CAAAAGGTTTTTATCAAAAATATGGCTTTCGCTGTTTTGAAGATAGTGAACATAAACTCTTTATCACCATAGCGGATGTG
CGGGCCAGCTTTGGCTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT243448 WP_001297137.1 NZ_CP096834:1568760-1569071 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT243448 NZ_OX352320:548454-548726 [Rheinheimera sp. MM224]
ATGACGACTACACCACCTCGAATCACGGCTCGGGTTGATGCTGAAACCCAAAACTTACTGTCTAAGGCTGCAGCTATAAC
TGGGGTTTCGAGCATCAATTCCTTTGTACTCAGCGCGGCGGTTGAGAAAGCCAAACAAATCATTGAGCGTGAGCAAACTC
TGAAGCTTTCTCAGCGTGATGCCATGTTATTGATGGACGCGCTTGATAACCCGGCGACTGTACATCCTCATCTGGCTGCA
GCTTTTGCACGTTATGAAGGCAAAGTTCAATAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3