243447

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1534681..1535299 Replicon chromosome
Accession NZ_CP096834
Organism Escherichia coli strain PM4-5

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MYF75_RS09215 Protein ID WP_001291435.1
Coordinates 1535081..1535299 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MYF75_RS09210 Protein ID WP_000344800.1
Coordinates 1534681..1535055 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF75_RS09200 (1529770) 1529770..1530963 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MYF75_RS09205 (1530986) 1530986..1534135 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
MYF75_RS09210 (1534681) 1534681..1535055 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MYF75_RS09215 (1535081) 1535081..1535299 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MYF75_RS09220 (1535471) 1535471..1536022 + 552 WP_000102564.1 maltose O-acetyltransferase -
MYF75_RS09225 (1536138) 1536138..1536608 + 471 WP_000136192.1 YlaC family protein -
MYF75_RS09230 (1536772) 1536772..1538322 + 1551 WP_089633415.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MYF75_RS09235 (1538364) 1538364..1538717 - 354 WP_000878141.1 DUF1428 family protein -
MYF75_RS09245 (1539096) 1539096..1539407 + 312 WP_000409911.1 MGMT family protein -
MYF75_RS09250 (1539438) 1539438..1540010 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T243447 WP_001291435.1 NZ_CP096834:1535081-1535299 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T243447 NZ_OX352319:3213193-3213564 [Pseudomonas sp. MM227]
ATGGTGAGGTCTCTGTTTGATACCAATATTCTGATCGACCACCTATCTGGTAGACCCTTGGCCGAGCAGGTTATCCACGC
TGCACACAGACCCATGATCAGCTTGATCACGTACATGGAGGTTTTAGGGGGTGCAGGTGATGCCAAGGAGGCCGACACCC
TGCGCCATTGGATGGGACAGCAATTCGAAGTGGTACCGGTGTCGCTCGATATCGCCCATGAGGCTGTCTTGCTGCGCCAG
CAGCGCCGCATCGAATTGCCCGACGCGATCATTTGGGCAAGCTCGCGCCTGGCAGGCGCCGTTTTGGTGACACGCAACAC
CAAGGATTTCCCCGAGACCGAGCTGGACGTGCGTGTGCCGTACCACCTGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT243447 WP_000344800.1 NZ_CP096834:1534681-1535055 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT243447 NZ_OX352319:3212972-3213199 [Pseudomonas sp. MM227]
ATGAGCCGGATTCTTGCCGACCTCACCCCCAAGCAACTTGAAGACCTTAACCAACTGGCATCGGCCTTGGACACGTCGCG
CGCAGCCCTGATCCGGGAAGCGGTGGCCACCTTCGTTGCGGTCAAGAATGAGGAGTTGAAGCACCCCGCCCCGGACGTTT
TCGGGCTGTGGAGGGATCGCGCCGTGGACGGCCTGGCTTATGAGCAGGAGCTGCGCAACGAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References