Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 487438..488076 Replicon chromosome
Accession NZ_CP096834
Organism Escherichia coli strain PM4-5

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag MYF75_RS04060 Protein ID WP_000813794.1
Coordinates 487900..488076 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag MYF75_RS04055 Protein ID WP_001270286.1
Coordinates 487438..487854 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF75_RS04035 (482590) 482590..483531 - 942 WP_001251315.1 ABC transporter permease -
MYF75_RS04040 (483532) 483532..484545 - 1014 WP_000220411.1 ABC transporter ATP-binding protein -
MYF75_RS04045 (484563) 484563..485708 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
MYF75_RS04050 (485953) 485953..487359 - 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
MYF75_RS04055 (487438) 487438..487854 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
MYF75_RS04060 (487900) 487900..488076 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
MYF75_RS04065 (488298) 488298..488528 + 231 WP_000494244.1 YncJ family protein -
MYF75_RS04070 (488620) 488620..490581 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
MYF75_RS04075 (490654) 490654..491190 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
MYF75_RS04080 (491282) 491282..492457 + 1176 WP_001236258.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T243446 WP_000813794.1 NZ_CP096834:c488076-487900 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T243446 NZ_OX352319:c1787539-1787273 [Pseudomonas sp. MM227]
ATGATTCGAAGCTTTGCATGCGCCGATACGGAAGCACTTTTCACCACCGGCAGGACAAGGCGCTGGGCAGACATCAAGTC
AGTCGCGGAGCGTAAACTGGCCGCACTGCATGCTGCGAAAGAGCTTCGAGACTTGCGCTCACCGCCCGGAAACCGGCTTC
ACACCTTGGAAGGTGACAGAGCCGGCCAGCATAACATGCAGTGGCGGGTATGCTTCATATGGACCCAGAACGGCCCTGAA
CACGTTGAGATTGTCGACTATCACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT243446 WP_001270286.1 NZ_CP096834:c487854-487438 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT243446 NZ_OX352319:c1787264-1786968 [Pseudomonas sp. MM227]
ATGTTCAAGAATGGCATGCGTCCCATACATCCCGGCGAGATTCTTCGCGAGGATTTCCAGCACGAAATGGGCTTCAGTGC
CGCTGCTCTGGCCCGTGCTTTAGGCGTATCTACGCCGACCGTGAATAACATTCTGCTTGAGCGTGGTGGCGTATCGGCTG
ACATGGCCTTGCGCTTGGCCGTCTGCCTGGATACCACTGCAGAGTTCTGGCTCAACCTGCAAACAGCGTACGAGTTACGC
AAGGCAGAGCTGGAGCATGGAGATGCGATTGCAGCGGTACAGCGCCTGGTTGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References