Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 240794..241436 Replicon chromosome
Accession NZ_CP096827
Organism Bacillus anthracis strain CVCC40205

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag M1D19_RS01325 Protein ID WP_000635963.1
Coordinates 241086..241436 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag M1D19_RS01320 Protein ID WP_000004570.1
Coordinates 240794..241081 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1D19_RS01295 (M1D19_01295) 236110..237072 + 963 WP_000961037.1 UV DNA damage repair endonuclease UvsE -
M1D19_RS01300 (M1D19_01300) 237065..237637 - 573 WP_000906916.1 rhomboid family intramembrane serine protease -
M1D19_RS01305 (M1D19_01305) 237730..238089 + 360 WP_000635040.1 holo-ACP synthase -
M1D19_RS01310 (M1D19_01310) 238246..239196 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
M1D19_RS01315 (M1D19_01315) 239315..240484 + 1170 WP_000390596.1 alanine racemase -
M1D19_RS01320 (M1D19_01320) 240794..241081 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
M1D19_RS01325 (M1D19_01325) 241086..241436 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M1D19_RS01330 (M1D19_01330) 241504..243672 + 2169 WP_000426225.1 Tex family protein -
M1D19_RS01335 (M1D19_01335) 243730..243846 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
M1D19_RS01340 (M1D19_01340) 244042..244500 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T243431 WP_000635963.1 NZ_CP096827:241086-241436 [Bacillus anthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T243431 NZ_OX344719:122639-122791 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT243431 WP_000004570.1 NZ_CP096827:240794-241081 [Bacillus anthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT243431 NZ_OX344719:122015-122614 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References