Detailed information of TA system TA243421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4921359..4922947
• Replicon: chromosome
• Accession: NZ_CP096821
• Organism: Pectobacterium sp. 21LCBS03

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: MYB54_RS21920
• Protein ID: WP_248721356.1
• Coordinates: 4921628..4922947 (+)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: MYB54_RS21915
• Protein ID: WP_103943261.1
• Coordinates: 4921359..4921628 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYB54_RS21885 (MYB54_21885)	4916397..4917056	+	660	WP_014917137.1	DUF799 domain-containing protein	-
MYB54_RS21890 (MYB54_21890)	4917179..4918681	-	1503	WP_248721354.1	MFS transporter	-
MYB54_RS21895 (MYB54_21895)	4918785..4919336	+	552	WP_039551752.1	TetR/AcrR family transcriptional regulator	-
MYB54_RS21900 (MYB54_21900)	4919367..4919777	-	411	WP_094370746.1	helix-turn-helix domain-containing protein	-
MYB54_RS21905 (MYB54_21905)	4919920..4920771	+	852	WP_248721355.1	SDR family oxidoreductase	-
MYB54_RS21910 (MYB54_21910)	4920951..4921166	+	216	Protein_4278	alpha/beta hydrolase	-
MYB54_RS21915 (MYB54_21915)	4921359..4921628	+	270	WP_103943261.1	helix-turn-helix domain-containing protein	Antitoxin
MYB54_RS21920 (MYB54_21920)	4921628..4922947	+	1320	WP_248721356.1	type II toxin-antitoxin system HipA family toxin	Toxin
MYB54_RS21925 (MYB54_21925)	4922980..4924344	-	1365	WP_039281388.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
MYB54_RS21930 (MYB54_21930)	4924474..4926108	-	1635	WP_010281240.1	membrane protein insertase YidC	-
MYB54_RS21935 (MYB54_21935)	4926111..4926368	-	258	WP_010281237.1	membrane protein insertion efficiency factor YidD	-
MYB54_RS21940 (MYB54_21940)	4926332..4926691	-	360	WP_010281235.1	ribonuclease P protein component	-
MYB54_RS21945 (MYB54_21945)	4926710..4926850	-	141	WP_005976668.1	50S ribosomal protein L34	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-66)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49269.56 Da
• Isoelectric Point: 6.9673

>T243421 WP_248721356.1 NZ_CP096821:4921628-4922947 [Pectobacterium sp. 21LCBS03]
MAALDVYMNGYRVGILTKTGSGTHHFSYDANWLGLPGSRPISLSMPLRHQPYQGDEVYNFFDNLLPDNPDIRRRIVARHH
TDSTQPFDLLAKVGQDSVGALQLVPQGTPVHDIKKIEYKTLSEQQLENILTGYLSDAPLGMIDTEDDFRISIAGAQEKTA
LLYLDDRWCLPLNATPTTHIIKLPIGKIESHSYSIDMSDSVENEYLCLLIAKAFGLPVPHCFMINAGKIKALAVERFDRK
YASDVSWIMRLPQEDFCQVLNVPSALKYENHGGPGISAIMSFLLGSVYPDRDRYRFMQAQVLFWLLAATDGHAKNFSLFI
ESEGRYRLTPFYDILSMYPAMGGRGIDRREAKMAMGLTGSRGKKYAIEQIFPRHFFQTAKVVGFARESMENILAEFAQTV
DTVMTTVGSKLPVDFPTHISEAILAGLQARSRRLMSGWA

Nucleotide

• Length: 1320 bp

>T243421 NZ_OX337252:2041319-2041421 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10062.40 Da
• Isoelectric Point: 9.7281

>AT243421 WP_103943261.1 NZ_CP096821:4921359-4921628 [Pectobacterium sp. 21LCBS03]
MMKITNSKQLSSYLKDVRITQKLSQGKVASKVGIRQDTVSSFEQHPDSTKLETFFKILSALNLELTVTPRNVDTTNNEAS
VASSWKEEW

Nucleotide

• Length: 270 bp

>AT243421 NZ_OX337252:c2041428-2041283 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT