Detailed information of TA system TA024342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 487735..488572
• Replicon: chromosome
• Accession: NC_013364
• Organism: Escherichia coli O111:H- str. 11128

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: ECO111_RS02365
• Protein ID: WP_000227784.1
• Coordinates: 487735..488277 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: ECO111_RS02370
• Protein ID: WP_001297137.1
• Coordinates: 488261..488572 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO111_RS02345	483274..484185	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
ECO111_RS02350	484353..484844	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
ECO111_RS02355	484972..486336	-	1365	WP_001000988.1	MFS transporter	-
ECO111_RS02360	486744..487679	+	936	WP_001345016.1	sel1 repeat family protein	-
ECO111_RS02365	487735..488277	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
ECO111_RS02370	488261..488572	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
ECO111_RS02375	488757..489647	-	891	WP_000971336.1	heme o synthase	-
ECO111_RS02380	489659..489988	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
ECO111_RS02385	489988..490602	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
ECO111_RS02390	490592..492583	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
ECO111_RS02395	492605..493552	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T24342 WP_000227784.1 NC_013364:c488277-487735 [Escherichia coli O111:H- str. 11128]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T24342 NC_013364:c488277-487735 [Escherichia coli O111:H- str. 11128]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT24342 WP_001297137.1 NC_013364:c488572-488261 [Escherichia coli O111:H- str. 11128]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT24342 NC_013364:c488572-488261 [Escherichia coli O111:H- str. 11128]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3