Detailed information of TA system TA243413

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1742248..1742973
• Replicon: chromosome
• Accession: NZ_CP096821
• Organism: Pectobacterium sp. 21LCBS03

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A0M2F4J3
• Locus tag: MYB54_RS07770
• Protein ID: WP_039311757.1
• Coordinates: 1742653..1742973 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: MYB54_RS07765
• Protein ID: WP_248721775.1
• Coordinates: 1742248..1742583 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYB54_RS07730 (MYB54_07730)	1737271..1737858	-	588	WP_248721770.1	inovirus-type Gp2 protein	-
MYB54_RS07735 (MYB54_07735)	1738233..1738901	+	669	WP_248721771.1	hypothetical protein	-
MYB54_RS07740 (MYB54_07740)	1738996..1739883	+	888	WP_248721772.1	50S ribosome-binding GTPase	-
MYB54_RS07745 (MYB54_07745)	1740067..1740225	+	159	WP_155242703.1	hypothetical protein	-
MYB54_RS07750 (MYB54_07750)	1740372..1741205	+	834	WP_248721773.1	hypothetical protein	-
MYB54_RS07755 (MYB54_07755)	1741262..1741612	+	351	WP_116238250.1	hypothetical protein	-
MYB54_RS07760 (MYB54_07760)	1741735..1742208	+	474	WP_248721774.1	DNA repair protein RadC	-
MYB54_RS07765 (MYB54_07765)	1742248..1742583	+	336	WP_248721775.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MYB54_RS07770 (MYB54_07770)	1742653..1742973	+	321	WP_039311757.1	TA system toxin CbtA family protein	Toxin
MYB54_RS07775 (MYB54_07775)	1743091..1743924	+	834	WP_248721776.1	DUF4942 domain-containing protein	-
MYB54_RS07780 (MYB54_07780)	1744018..1744248	+	231	WP_248721777.1	type II toxin-antitoxin system VapB family antitoxin	-
MYB54_RS07785 (MYB54_07785)	1744248..1744652	+	405	WP_198339801.1	tRNA(fMet)-specific endonuclease VapC	-
MYB54_RS07790 (MYB54_07790)	1745131..1746144	-	1014	WP_248721778.1	CAR family subclass B3 metallo-beta-lactamase	-
MYB54_RS07795 (MYB54_07795)	1746256..1747155	+	900	WP_205563835.1	LysR family transcriptional regulator	-
MYB54_RS07800 (MYB54_07800)	1747210..1747566	-	357	WP_052235227.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	flhD / flhC / motA / motB / cheA / cheW / cheD / cheR / cheB	1714585..1763889	49304

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (8-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11989.69 Da
• Isoelectric Point: 5.0493

>T243413 WP_039311757.1 NZ_CP096821:1742653-1742973 [Pectobacterium sp. 21LCBS03]
MQTTPAIPPREDNPCPSPITVWQQLLTYLLEKHYGLTLNDTPFGEENVIQAHIDAGVTLVNAVNFLVEKYELVRIDRNGF
NWQEQSPFLTAVDVLRARRATGLLKA

Nucleotide

• Length: 321 bp

>T243413 NZ_OX281345:1528175-1528573 [Thermococcus nautili]
GTGATAGTAATTGACGCCTCCGTTCTGGCGAAGTACGTCCTCATCGAGCCGGGCTGGGAGGAAGTAGAGAAACTCCTGAT
GGGCGACGCCGTTTCGCTGGACTACGCCCTCGCGGAAGTCTCCAACGCCCTTTGGAAGCACCACGTCCTGTACGGGGAGA
TATCGCGGGAGGAATTTGAGAGACGGTCAAAGGTAATAGACTTCCTCCCGAACGTCATTCTCCTCGAGGACGGGACCAAC
TACTTAACGGAAGCAAGGAAAATAGCGGTCGAGCACCGCATACCCATCTACGACGCCCTCTACATCGCCCAGGCCCTCAA
GTACGGAAAGCTCGCGACGGCCGATGAGAGGCAGGGAAAAATAGCCGAAAAGCTCGGCGTTGAGGTGGTTTACCTCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12488.15 Da
• Isoelectric Point: 7.1882

>AT243413 WP_248721775.1 NZ_CP096821:1742248-1742583 [Pectobacterium sp. 21LCBS03]
MSSIEASEWGLKCAVTPRFGARLVQEGNRLHYLADRASFIGTFSKAEARNLEHVFPEQIKQLERKLRTGELNPRQQNCVT
LHCNEWICEADTLGSVGYVYIAIYPSSAATQ

Nucleotide

• Length: 336 bp

>AT243413 NZ_OX281345:1527967-1528188 [Thermococcus nautili]
ATGGCTGTCATAAGCGTCCGCGTTCCCGACGAGCTTAAGGAGAAGATGAAGAAGTACGACATCAACTGGAGCGAGGAGAT
ACGGCGGTTCATAGCGACTAGGATAAGCGAGGAGGAAAAAAAGCGGACACTCGAGATGATGCACGAGCTGCTCGCGGGTG
GAACGCCAGCCAAGGCAGGAACCGCAAAAAGGTACGTGAGGGAGGACCGTGATAGTAATTGA

Structures

Toxin

• AlphaFold DB: A0A0M2F4J3