Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 120978..121714 Replicon plasmid unnamed1
Accession NZ_CP096815
Organism Klebsiella pneumoniae strain Mu1413

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag M1R63_RS26405 Protein ID WP_003026803.1
Coordinates 120978..121460 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag M1R63_RS26410 Protein ID WP_003026799.1
Coordinates 121448..121714 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1R63_RS26385 116845..116976 - 132 WP_004218042.1 hypothetical protein -
M1R63_RS26390 (M1R63_26360) 117137..118483 - 1347 WP_020314316.1 ISNCY family transposase -
M1R63_RS26395 (M1R63_26365) 118713..119345 + 633 WP_001567369.1 hypothetical protein -
M1R63_RS26400 (M1R63_26370) 119374..120777 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
M1R63_RS26405 (M1R63_26375) 120978..121460 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
M1R63_RS26410 (M1R63_26380) 121448..121714 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
M1R63_RS26415 (M1R63_26385) 121890..122144 - 255 WP_004152108.1 hypothetical protein -
M1R63_RS26420 (M1R63_26390) 122220..122477 - 258 WP_004152107.1 hypothetical protein -
M1R63_RS26425 (M1R63_26395) 122526..122729 - 204 WP_004152106.1 HHA domain-containing protein -
M1R63_RS26430 (M1R63_26400) 122763..123131 - 369 WP_004152105.1 hypothetical protein -
M1R63_RS26435 (M1R63_26405) 123175..123669 - 495 WP_004152104.1 DNA-binding protein -
M1R63_RS26440 (M1R63_26410) 123700..124275 - 576 WP_004152103.1 hypothetical protein -
M1R63_RS26445 (M1R63_26415) 124263..124514 - 252 WP_284659849.1 hypothetical protein -
M1R63_RS26450 (M1R63_26420) 124539..125243 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
M1R63_RS26455 (M1R63_26425) 125289..125711 - 423 Protein_137 alpha/beta hydrolase-fold protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaSHV-28 / catA1 / dfrA12 / aadA2 / tet(D) / aac(6')-Ib - 1..207747 207747
- inside IScluster/Tn tet(D) / aac(6')-Ib - 115609..140740 25131


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T243408 WP_003026803.1 NZ_CP096815:c121460-120978 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T243408 NZ_OX216966:5302833-5303021 [Paenibacillus dendritiformis]
TTGATAAAGCGTACTCATCAAGAGAAATTAATCCTTAAATCAGATGGTTGGGTACATAAGAATACAGAAGGTGACCACTA
CCACTTCGTTCATCCAACCAAAAAGGGAAAGGTTTCCGTACCTCATCCCAAGAAAGACATAAAACGGAGAACGACAAAAA
GCATCTTCAAGCAGGCGGGGCTGATTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT243408 WP_003026799.1 NZ_CP096815:c121714-121448 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT243408 NZ_OX216966:5303052-5303465 [Paenibacillus dendritiformis]
ATGAGCAAGTACATTTATCCTGCTGTGTTCCATCCGGGCGAGCCAGACGAAGGCGGTTATACCGTAACCTTCCCTGACTT
GCCGGGCTGCATTTCCGAAGGCGATACCCTCGACGAAGCATTGCATATGGCGAAAGACGCCCTTGGTGGACATCTCTACT
TGATGGAAGAGGATAAAGACGATATCCCGCAACCAACGAAGCCGAACGCAATTCAATTGGAAGACGATGAATTTGTGTCT
CTCATTCAAGTGAGTACTGATTTCGTCCGCGAGCGCATCCGTAATAAGGCCATCAATAAGACACTCACAATTCCAAAATG
GCTAAACGATGCTGCTGCCGAAGAAGGGATCAACTTCTCTCAGACGCTTCAGGATGCATTGAAGGAGAAACTTGGTGTTA
AAGACTTCTCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References