Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5227053..5227678 Replicon chromosome
Accession NZ_CP096814
Organism Klebsiella pneumoniae strain Mu1413

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag M1R63_RS25405 Protein ID WP_002882817.1
Coordinates 5227053..5227436 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M1R63_RS25410 Protein ID WP_004150355.1
Coordinates 5227436..5227678 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1R63_RS25390 (5224419) 5224419..5225321 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M1R63_RS25395 (5225318) 5225318..5225953 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M1R63_RS25400 (5225950) 5225950..5226879 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M1R63_RS25405 (5227053) 5227053..5227436 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
M1R63_RS25410 (5227436) 5227436..5227678 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M1R63_RS25415 (5227883) 5227883..5228800 + 918 WP_004181612.1 alpha/beta hydrolase -
M1R63_RS25420 (5228814) 5228814..5229755 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M1R63_RS25425 (5229800) 5229800..5230237 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M1R63_RS25430 (5230234) 5230234..5231094 - 861 WP_004146232.1 virulence factor BrkB family protein -
M1R63_RS25435 (5231088) 5231088..5231687 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T243407 WP_002882817.1 NZ_CP096814:c5227436-5227053 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T243407 NZ_OX030730:2224-2499 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT243407 WP_004150355.1 NZ_CP096814:c5227678-5227436 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT243407 NZ_OX030730:1940-2224 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTCATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References