Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4626109..4626919 Replicon chromosome
Accession NZ_CP096814
Organism Klebsiella pneumoniae strain Mu1413

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag M1R63_RS22570 Protein ID WP_004178461.1
Coordinates 4626109..4626642 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag M1R63_RS22575 Protein ID WP_002887278.1
Coordinates 4626653..4626919 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1R63_RS22565 (4624940) 4624940..4626061 + 1122 WP_002887282.1 cupin domain-containing protein -
M1R63_RS22570 (4626109) 4626109..4626642 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
M1R63_RS22575 (4626653) 4626653..4626919 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
M1R63_RS22580 (4627022) 4627022..4628455 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
M1R63_RS22585 (4628445) 4628445..4629128 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
M1R63_RS22590 (4629300) 4629300..4630685 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
M1R63_RS22595 (4630703) 4630703..4631047 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T243403 WP_004178461.1 NZ_CP096814:c4626642-4626109 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T243403 NZ_OX030729:c33958-33824 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT243403 WP_002887278.1 NZ_CP096814:c4626919-4626653 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT243403 NZ_OX030729:34003-34063 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References