Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 4384663..4385342 Replicon chromosome
Accession NZ_CP096814
Organism Klebsiella pneumoniae strain Mu1413

Toxin (Protein)


Gene name ykfI Uniprot ID A0A0C7K7A4
Locus tag M1R63_RS21360 Protein ID WP_020324801.1
Coordinates 4384663..4385004 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0C7KEL2
Locus tag M1R63_RS21365 Protein ID WP_020324792.1
Coordinates 4385025..4385342 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1R63_RS21340 (4380964) 4380964..4382805 + 1842 WP_004174339.1 RNA polymerase sigma factor RpoD -
M1R63_RS21345 (4382905) 4382905..4383411 - 507 WP_002917636.1 G/U mismatch-specific DNA glycosylase -
M1R63_RS21355 (4383714) 4383714..4384547 - 834 WP_020324805.1 DUF4942 domain-containing protein -
M1R63_RS21360 (4384663) 4384663..4385004 - 342 WP_020324801.1 TA system toxin CbtA family protein Toxin
M1R63_RS21365 (4385025) 4385025..4385342 - 318 WP_020324792.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
M1R63_RS21370 (4385355) 4385355..4385582 - 228 WP_020324796.1 DUF987 domain-containing protein -
M1R63_RS21375 (4385591) 4385591..4386067 - 477 WP_020324797.1 RadC family protein -
M1R63_RS21380 (4386083) 4386083..4386541 - 459 WP_020324813.1 antirestriction protein -
M1R63_RS21385 (4386644) 4386644..4386883 - 240 WP_020324820.1 DUF905 domain-containing protein -
M1R63_RS21390 (4386976) 4386976..4390128 - 3153 WP_022631414.1 AIDA repeat-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-111)

Antitoxin

Antitoxin CbeA

(5-103)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12818.91 Da        Isoelectric Point: 9.1484

>T243402 WP_020324801.1 NZ_CP096814:c4385004-4384663 [Klebsiella pneumoniae]
MKTLPAIISRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFCDETVIQEHIDAGITLADAVNFLVEKYGLVRIDRNGF
VWQEQSPYLRAVDILRARQATGLLRQSRNNAIR

Download         Length: 342 bp

>T243402 NZ_OX030728:c5210671-5210285 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11915.51 Da        Isoelectric Point: 6.6246

>AT243402 WP_020324792.1 NZ_CP096814:c4385342-4385025 [Klebsiella pneumoniae]
MSNITWGLQRDTTPRLGARLVQEGNQLHYLADRASMTGKFSDAECRKLDETFPHFISQMESMLITGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAVYPIQR

Download         Length: 318 bp

>AT243402 NZ_OX030728:c5210295-5209966 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7K7A4

Antitoxin

Source ID Structure
AlphaFold DB A0A0C7KEL2

References