Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4236726..4237383 Replicon chromosome
Accession NZ_CP096814
Organism Klebsiella pneumoniae strain Mu1413

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag M1R63_RS20610 Protein ID WP_002916310.1
Coordinates 4236726..4237136 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag M1R63_RS20615 Protein ID WP_002916312.1
Coordinates 4237117..4237383 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1R63_RS20590 (4232726) 4232726..4234459 - 1734 WP_004149758.1 single-stranded-DNA-specific exonuclease RecJ -
M1R63_RS20595 (4234465) 4234465..4235178 - 714 WP_002916301.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
M1R63_RS20600 (4235201) 4235201..4236097 - 897 WP_004144729.1 site-specific tyrosine recombinase XerD -
M1R63_RS20605 (4236198) 4236198..4236719 + 522 WP_004144730.1 flavodoxin FldB -
M1R63_RS20610 (4236726) 4236726..4237136 - 411 WP_002916310.1 protein YgfX Toxin
M1R63_RS20615 (4237117) 4237117..4237383 - 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
M1R63_RS20620 (4237629) 4237629..4238612 + 984 WP_020324927.1 tRNA-modifying protein YgfZ -
M1R63_RS20625 (4238763) 4238763..4239422 - 660 WP_002916317.1 hemolysin III family protein -
M1R63_RS20630 (4239586) 4239586..4239897 - 312 WP_004144734.1 N(4)-acetylcytidine aminohydrolase -
M1R63_RS20635 (4239947) 4239947..4240675 + 729 WP_002916321.1 MurR/RpiR family transcriptional regulator -
M1R63_RS20640 (4240794) 4240794..4242227 + 1434 WP_004185559.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T243401 WP_002916310.1 NZ_CP096814:c4237136-4236726 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T243401 NZ_OX030728:c4786540-4786322 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT243401 WP_002916312.1 NZ_CP096814:c4237383-4237117 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT243401 NZ_OX030728:c4786940-4786566 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References