Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1069470..1070089 Replicon chromosome
Accession NZ_CP096814
Organism Klebsiella pneumoniae strain Mu1413

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag M1R63_RS05150 Protein ID WP_002892050.1
Coordinates 1069470..1069688 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag M1R63_RS05155 Protein ID WP_002892066.1
Coordinates 1069715..1070089 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1R63_RS05115 (1065517) 1065517..1065780 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
M1R63_RS05120 (1065780) 1065780..1065920 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
M1R63_RS05125 (1065917) 1065917..1066615 - 699 WP_002892021.1 GNAT family protein -
M1R63_RS05130 (1066716) 1066716..1068167 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
M1R63_RS05135 (1068142) 1068142..1068612 - 471 WP_002892026.1 YlaC family protein -
M1R63_RS05140 (1068633) 1068633..1068773 + 141 WP_004147370.1 hypothetical protein -
M1R63_RS05145 (1068745) 1068745..1069311 - 567 WP_002892030.1 maltose O-acetyltransferase -
M1R63_RS05150 (1069470) 1069470..1069688 - 219 WP_002892050.1 HHA domain-containing protein Toxin
M1R63_RS05155 (1069715) 1069715..1070089 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
M1R63_RS05160 (1070575) 1070575..1073721 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
M1R63_RS05165 (1073744) 1073744..1074937 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T243391 WP_002892050.1 NZ_CP096814:c1069688-1069470 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T243391 NZ_OX030728:2512422-2512799 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT243391 WP_002892066.1 NZ_CP096814:c1070089-1069715 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT243391 NZ_OX030728:2511965-2512345 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCGCCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References