Detailed information of TA system TA243389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 5935746..5937004
• Replicon: chromosome
• Accession: NZ_CP096813
• Organism: Pseudomonas aeruginosa strain 8D

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: M1Q10_RS27555
• Protein ID: WP_003112528.1
• Coordinates: 5935746..5936501 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: M1Q10_RS27560
• Protein ID: WP_003086329.1
• Coordinates: 5936636..5937004 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1Q10_RS27545 (M1Q10_27545)	5930919..5933462	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
M1Q10_RS27550 (M1Q10_27550)	5933617..5934978	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
M1Q10_RS27555 (M1Q10_27555)	5935746..5936501	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
M1Q10_RS27560 (M1Q10_27560)	5936636..5937004	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
M1Q10_RS27565 (M1Q10_27565)	5937077..5938363	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
M1Q10_RS27570 (M1Q10_27570)	5938419..5939912	-	1494	WP_003086325.1	aldehyde dehydrogenase family protein	-
M1Q10_RS27575 (M1Q10_27575)	5940087..5940563	+	477	WP_003112531.1	hypothetical protein	-
M1Q10_RS27580 (M1Q10_27580)	5940566..5940841	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T243389 WP_003112528.1 NZ_CP096813:c5936501-5935746 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T243389 NZ_OX030728:c2188264-2187929 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT243389 WP_003086329.1 NZ_CP096813:c5937004-5936636 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT243389 NZ_OX030728:c2188512-2188264 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7