Detailed information of TA system TA243388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 5021430..5022651
• Replicon: chromosome
• Accession: NZ_CP096813
• Organism: Pseudomonas aeruginosa strain 8D

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: M1Q10_RS23245
• Protein ID: WP_003102740.1
• Coordinates: 5022073..5022651 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: M1Q10_RS23240
• Protein ID: WP_003088112.1
• Coordinates: 5021430..5021984 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1Q10_RS23215 (M1Q10_23215)	5016998..5017549	+	552	WP_004343115.1	XRE family transcriptional regulator	-
M1Q10_RS23220 (M1Q10_23220)	5017546..5017944	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
M1Q10_RS23225 (M1Q10_23225)	5017957..5018280	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
M1Q10_RS23230 (M1Q10_23230)	5018611..5019072	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
M1Q10_RS23235 (M1Q10_23235)	5019077..5021272	+	2196	WP_010794059.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
M1Q10_RS23240 (M1Q10_23240)	5021430..5021984	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
M1Q10_RS23245 (M1Q10_23245)	5022073..5022651	+	579	WP_003102740.1	HD domain-containing protein	Toxin
M1Q10_RS23250 (M1Q10_23250)	5022760..5023947	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
M1Q10_RS23255 (M1Q10_23255)	5023937..5026108	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
M1Q10_RS23260 (M1Q10_23260)	5026098..5027375	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T243388 WP_003102740.1 NZ_CP096813:5022073-5022651 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T243388 NZ_OX030728:c2115705-2115394 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT243388 WP_003088112.1 NZ_CP096813:5021430-5021984 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT243388 NZ_OX030728:c2115397-2114978 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07