Detailed information of TA system TA243385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1523868..1524373
• Replicon: chromosome
• Accession: NZ_CP096813
• Organism: Pseudomonas aeruginosa strain 8D

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: M1Q10_RS07060
• Protein ID: WP_003083773.1
• Coordinates: 1523868..1524149 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: M1Q10_RS07065
• Protein ID: WP_003083775.1
• Coordinates: 1524146..1524373 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1Q10_RS07035 (M1Q10_07035)	1519119..1520468	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
M1Q10_RS07040 (M1Q10_07040)	1520517..1521203	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
M1Q10_RS07045 (M1Q10_07045)	1521304..1522038	+	735	WP_003117208.1	GntR family transcriptional regulator	-
M1Q10_RS07050 (M1Q10_07050)	1522218..1522628	+	411	WP_003101225.1	aegerolysin family protein	-
M1Q10_RS07055 (M1Q10_07055)	1522660..1523568	-	909	WP_010793581.1	LysR family transcriptional regulator	-
M1Q10_RS07060 (M1Q10_07060)	1523868..1524149	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
M1Q10_RS07065 (M1Q10_07065)	1524146..1524373	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
M1Q10_RS07070 (M1Q10_07070)	1524549..1525169	-	621	WP_003101226.1	hypothetical protein	-
M1Q10_RS07075 (M1Q10_07075)	1525270..1525770	+	501	WP_003101228.1	LEA type 2 family protein	-
M1Q10_RS07080 (M1Q10_07080)	1525843..1526184	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
M1Q10_RS07085 (M1Q10_07085)	1526266..1527693	-	1428	WP_003083784.1	GABA permease	-
M1Q10_RS07090 (M1Q10_07090)	1527862..1529355	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T243385 WP_003083773.1 NZ_CP096813:c1524149-1523868 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T243385 NZ_OX030728:604320-604496 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT243385 WP_003083775.1 NZ_CP096813:c1524373-1524146 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT243385 NZ_OX030728:604542-604958 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9