243380

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 235306..236216 Replicon plasmid pBio19
Accession NZ_CP096811
Organism Klebsiella pneumoniae strain Bio19

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag MOV22_RS28085 Protein ID WP_004026354.1
Coordinates 235746..236216 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag MOV22_RS28080 Protein ID WP_004026357.1
Coordinates 235306..235749 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOV22_RS28055 (MOV22_28055) 230601..231572 + 972 WP_023287152.1 ParB/RepB/Spo0J family plasmid partition protein -
MOV22_RS28060 (MOV22_28060) 232423..232651 - 229 Protein_228 type II toxin-antitoxin system Phd/YefM family antitoxin -
MOV22_RS28065 (MOV22_28065) 232730..233361 - 632 Protein_229 DUF4113 domain-containing protein -
MOV22_RS28070 (MOV22_28070) 233498..234195 + 698 WP_223364782.1 IS1 family transposase -
MOV22_RS28075 (MOV22_28075) 234210..235205 + 996 Protein_231 translesion error-prone DNA polymerase V subunit UmuC -
MOV22_RS28080 (MOV22_28080) 235306..235749 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
MOV22_RS28085 (MOV22_28085) 235746..236216 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
MOV22_RS28090 (MOV22_28090) 236671..236859 - 189 WP_024198076.1 hypothetical protein -
MOV22_RS28095 (MOV22_28095) 237471..237731 - 261 WP_004026352.1 hypothetical protein -
MOV22_RS28100 (MOV22_28100) 237937..238719 - 783 WP_248764260.1 alpha/beta hydrolase -
MOV22_RS28105 (MOV22_28105) 238960..239829 + 870 WP_001567390.1 DMT family transporter -
MOV22_RS28110 (MOV22_28110) 239963..241186 + 1224 WP_023287190.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 233692..234195 503
- inside Conjugative plasmid dfrA14 ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA 1..292530 292530


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T243380 WP_004026354.1 NZ_CP096811:235746-236216 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T243380 NZ_CP096811:235746-236216 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCTTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATTGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT243380 WP_004026357.1 NZ_CP096811:235306-235749 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT243380 NZ_CP096811:235306-235749 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACTTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References