243379

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 144325..145061 Replicon plasmid pBio19
Accession NZ_CP096811
Organism Klebsiella pneumoniae strain Bio19

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag MOV22_RS27560 Protein ID WP_003026803.1
Coordinates 144325..144807 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag MOV22_RS27565 Protein ID WP_003026799.1
Coordinates 144795..145061 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOV22_RS27540 (MOV22_27540) 139933..141366 - 1434 WP_004016055.1 restriction endonuclease -
MOV22_RS27545 (MOV22_27545) 141387..142190 - 804 WP_000619352.1 TIGR02391 family protein -
MOV22_RS27555 (MOV22_27555) 143528..143944 - 417 WP_077265827.1 hypothetical protein -
MOV22_RS27560 (MOV22_27560) 144325..144807 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
MOV22_RS27565 (MOV22_27565) 144795..145061 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
MOV22_RS27570 (MOV22_27570) 145306..145740 - 435 WP_001567367.1 cell envelope integrity protein TolA -
MOV22_RS27575 (MOV22_27575) 145787..146335 - 549 WP_001567366.1 thioredoxin fold domain-containing protein -
MOV22_RS27580 (MOV22_27580) 146787..147137 + 351 WP_004200891.1 hypothetical protein -
MOV22_RS28520 147237..147359 + 123 WP_254913144.1 hypothetical protein -
MOV22_RS27585 (MOV22_27585) 148362..148787 - 426 WP_248764259.1 DNA-binding protein -
MOV22_RS27590 (MOV22_27590) 149055..149537 - 483 WP_004210306.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA 1..292530 292530


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T243379 WP_003026803.1 NZ_CP096811:c144807-144325 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T243379 NZ_OX030703:c77804-77499 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT243379 WP_003026799.1 NZ_CP096811:c145061-144795 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT243379 NZ_OX030703:c78024-77806 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References