Detailed information of TA system TA243378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 123744..124387
• Replicon: plasmid pBio19
• Accession: NZ_CP096811
• Organism: Klebsiella pneumoniae strain Bio19

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A5Q9LNG2
• Locus tag: MOV22_RS27480
• Protein ID: WP_048983057.1
• Coordinates: 123744..124160 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: R4IT22
• Locus tag: MOV22_RS27485
• Protein ID: WP_016338373.1
• Coordinates: 124157..124387 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOV22_RS27460 (MOV22_27460)	118792..120900	-	2109	WP_016338368.1	peptidase domain-containing ABC transporter	-
MOV22_RS27465 (MOV22_27465)	120890..122167	-	1278	WP_016338369.1	HlyD family secretion protein	-
MOV22_RS27470 (MOV22_27470)	122826..123362	+	537	WP_223825639.1	hypothetical protein	-
MOV22_RS27475 (MOV22_27475)	123380..123703	+	324	WP_085842301.1	hypothetical protein	-
MOV22_RS27480 (MOV22_27480)	123744..124160	-	417	WP_048983057.1	type II toxin-antitoxin system VapC family toxin	Toxin
MOV22_RS27485 (MOV22_27485)	124157..124387	-	231	WP_016338373.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MOV22_RS27490 (MOV22_27490)	125032..126012	-	981	WP_000019403.1	IS5-like element IS5 family transposase	-
MOV22_RS27495 (MOV22_27495)	126058..126726	-	669	WP_032249507.1	LuxR C-terminal-related transcriptional regulator	-
MOV22_RS27500 (MOV22_27500)	126924..127892	-	969	WP_077265821.1	IS5 family transposase	-
MOV22_RS27505 (MOV22_27505)	128222..128518	+	297	WP_001542733.1	helix-turn-helix transcriptional regulator	-
MOV22_RS27510 (MOV22_27510)	128780..128968	-	189	Protein_117	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA14	ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA	1..292530	292530

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15022.52 Da
• Isoelectric Point: 9.2957

>T243378 WP_048983057.1 NZ_CP096811:c124160-123744 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T243378 NZ_OX030702:c25945-25529 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTGACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8751.77 Da
• Isoelectric Point: 4.3533

>AT243378 WP_016338373.1 NZ_CP096811:c124387-124157 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT243378 NZ_OX030702:c26172-25942 [Escherichia coli]
ATGAGAACCGTATCCATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAAGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACCGCATCATCCTGCGTCCCGTCCGCCCGGCCTGGGGCTCGTTCGCGCAGCTCG
AGAAAGCCGATCCGGACTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LNG2

Antitoxin

• AlphaFold DB: A0A5Q9LN61