Detailed information of TA system TA243377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 4014..4753
• Replicon: plasmid pBio19
• Accession: NZ_CP096811
• Organism: Klebsiella pneumoniae strain Bio19

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: MOV22_RS26945
• Protein ID: WP_021312536.1
• Coordinates: 4268..4753 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: MOV22_RS26940
• Protein ID: WP_049095044.1
• Coordinates: 4014..4280 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOV22_RS26925 (MOV22_26925)	740..1744	-	1005	WP_117256483.1	IS110 family transposase	-
MOV22_RS26930 (MOV22_26930)	1883..3252	+	1370	WP_087661561.1	IS3 family transposase	-
MOV22_RS26935 (MOV22_26935)	3453..3881	+	429	WP_004901287.1	GFA family protein	-
MOV22_RS26940 (MOV22_26940)	4014..4280	+	267	WP_049095044.1	DUF1778 domain-containing protein	Antitoxin
MOV22_RS26945 (MOV22_26945)	4268..4753	+	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
MOV22_RS26950 (MOV22_26950)	5046..6125	-	1080	WP_117256484.1	IS481-like element ISKpn28 family transposase	-
MOV22_RS26955 (MOV22_26955)	6197..6358	+	162	WP_004118124.1	type I toxin-antitoxin system Hok family toxin	-
MOV22_RS26960 (MOV22_26960)	6960..7934	+	975	WP_032719543.1	hypothetical protein	-
MOV22_RS26965 (MOV22_26965)	8131..8508	-	378	WP_032719541.1	hypothetical protein	-
MOV22_RS26970 (MOV22_26970)	8919..9131	+	213	WP_032427880.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA14	ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA	1..292530	292530
	-	inside	IScluster/Tn	-	-	740..6125	5385

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T243377 WP_021312536.1 NZ_CP096811:4268-4753 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T243377 NZ_OX030702:2041-2319 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCATATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
TGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10163.63 Da
• Isoelectric Point: 4.5256

>AT243377 WP_049095044.1 NZ_CP096811:4014-4280 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDELAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT243377 NZ_OX030702:1772-2041 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGGCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGCCAGCAACTTGAGCGCGGTGATGTGCTTTCCCATGACATGGTCGAATCCTCAGCTACCGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA