Detailed information of TA system TA243374

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 5235045..5235855
• Replicon: chromosome
• Accession: NZ_CP096810
• Organism: Klebsiella pneumoniae strain Bio19

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: MOV22_RS25440
• Protein ID: WP_040148415.1
• Coordinates: 5235045..5235578 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: MOV22_RS25445
• Protein ID: WP_002887278.1
• Coordinates: 5235589..5235855 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOV22_RS25410 (5230226)	5230226..5230768	+	543	WP_004145330.1	type I DNA topoisomerase	-
MOV22_RS25415 (5230773)	5230773..5231345	+	573	WP_002919132.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
MOV22_RS25420 (5231349)	5231349..5232167	+	819	WP_002919126.1	shikimate dehydrogenase	-
MOV22_RS25425 (5232164)	5232164..5232421	+	258	WP_002919125.1	DUF1488 domain-containing protein	-
MOV22_RS25430 (5232397)	5232397..5232951	-	555	WP_002919123.1	gamma carbonic anhydrase family protein	-
MOV22_RS25435 (5233876)	5233876..5234997	+	1122	WP_016946712.1	cupin domain-containing protein	-
MOV22_RS25440 (5235045)	5235045..5235578	-	534	WP_040148415.1	type II toxin-antitoxin system toxin KacT	Toxin
MOV22_RS25445 (5235589)	5235589..5235855	-	267	WP_002887278.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
MOV22_RS25450 (5235958)	5235958..5237391	-	1434	WP_087775173.1	Cu(+)/Ag(+) sensor histidine kinase	-
MOV22_RS25455 (5237381)	5237381..5238064	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
MOV22_RS25460 (5238237)	5238237..5239622	+	1386	WP_087775195.1	efflux transporter outer membrane subunit	-
MOV22_RS25465 (5239640)	5239640..5239984	+	345	WP_087775198.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19909.78 Da
• Isoelectric Point: 5.7068

>T243374 WP_040148415.1 NZ_CP096810:c5235578-5235045 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSRDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T243374 NZ_OX030701:c4112080-4111862 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT243374 WP_002887278.1 NZ_CP096810:c5235855-5235589 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT243374 NZ_OX030701:c4112480-4112106 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GLZ1