Detailed information of TA system TA243371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4865418..4866157
• Replicon: chromosome
• Accession: NZ_CP096810
• Organism: Klebsiella pneumoniae strain Bio19

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: MOV22_RS23395
• Protein ID: WP_021312536.1
• Coordinates: 4865418..4865903 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: MOV22_RS23400
• Protein ID: WP_049095044.1
• Coordinates: 4865891..4866157 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOV22_RS23370 (4861040)	4861040..4861252	-	213	WP_032427880.1	hypothetical protein	-
MOV22_RS23375 (4861663)	4861663..4862040	+	378	WP_032719541.1	hypothetical protein	-
MOV22_RS23380 (4862237)	4862237..4863211	-	975	WP_032719543.1	hypothetical protein	-
MOV22_RS23385 (4863813)	4863813..4863974	-	162	WP_004118124.1	type I toxin-antitoxin system Hok family toxin	-
MOV22_RS23390 (4864046)	4864046..4865125	+	1080	WP_117256484.1	IS481-like element ISKpn28 family transposase	-
MOV22_RS23395 (4865418)	4865418..4865903	-	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
MOV22_RS23400 (4865891)	4865891..4866157	-	267	WP_049095044.1	DUF1778 domain-containing protein	Antitoxin
MOV22_RS23405 (4866290)	4866290..4866718	-	429	WP_004901287.1	GFA family protein	-
MOV22_RS23415 (4868427)	4868427..4869431	+	1005	WP_117256483.1	IS110 family transposase	-
MOV22_RS23420 (4870074)	4870074..4870232	+	159	WP_248764200.1	IS1 family transposase	-
MOV22_RS23425 (4870247)	4870247..4870987	-	741	Protein_4616	filamentous hemagglutinin N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	4864046..4869431	5385

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T243371 WP_021312536.1 NZ_CP096810:c4865903-4865418 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T243371 NZ_OX030701:3502063-3502350 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10163.63 Da
• Isoelectric Point: 4.5256

>AT243371 WP_049095044.1 NZ_CP096810:c4866157-4865891 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDELAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT243371 NZ_OX030701:3502337-3502639 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA